The origin recognition complex (ORC) binds sites from which DNA replication is initiated. We address ORC binding selectivity in vivo by mapping ∼52,000 ORC2 binding sites throughout the human genome. The ORC binding profile is broader than those of sequence-specific transcription factors, suggesting that ORC is not bound or recruited to specific DNA sequences. Instead, ORC binds nonspecifically...

The Organic Rankine Cycle (ORC) is commonly accepted as a viable technology to convert from low medium temperature geothermal energy into electrical energy. In practice, the reference for converting electricity subcritical simple ORC system. Over time, plants with more complex configurations (architectures) have been developed. open literature, large number of advanced architectures or introduc...

Recently, a promising programming model called Orc has been proposed to support a structured way of orchestrating distributed web services. Orc is intuitive because it offers concise constructors to manage concurrent communication, time-outs, priorities, failure of sites or communication and so forth. The semantics of Orc is also precisely defined. However, there is no verification tool availab...

DNA replication, as with all macromolecular synthesis steps, is controlled in part at the level of initiation. Although the origin recognition complex (ORC) binds to origins ofDNAreplication, it does not solely determine their location. To initiate DNA replication ORC requires Cdc6 to target initiation to specific DNA sequences in chromosomes andwithCdt1 loads the ring-shaped mini-chromosome ma...

BACKGROUND This study aimed to investigate the relationship between oxidized regenerated cellulose (ORC) and mucosa healing in an experimental animal model. METHODS Fifteen adult Sprague-Dawley rats were randomly divided into three groups that underwent different wound treatments. In Group 1, no pharyngeal wound was created. In Group 2, the pharyngeal wound was sutured with Prolene only. In G...

For Orc, a language for orchestrating concurrent computations, a “token semantics” is presented. This semantic model preserves the original program text during evaluation, rather than reducing the given program to its evaluated value.

BACKGROUND The origin recognition complex (ORC) and the minichromosome maintenance (MCM) protein complex were initially discovered in yeast and shown to be essential for DNA replication. Homologues of ORC and MCM proteins exist in higher eukaryotes, including Xenopus. The Xenopus MCM proteins and the Xenopus homologues of Saccharomyces cerevisiae Orc 1p and Orc2p (XOrc1 and XOrc2) have recently...

In higher eukaryotes, the origin recognition complex (ORC) lacks sequence-specific DNA binding, and it remains unclear what other factors specify an origin of DNA replication. The Epstein-Barr virus origin of plasmid replication (OriP) recruits ORC, but the precise mechanism of ORC recruitment and origin activation is not clear. We now show that ORC is recruited selectively to the dyad symmetry...

In all eukaryotic cells, origins of DNA replication are characterized by the binding of the origin recognition complex (ORC). How ORC is positioned to sites where replication initiates is unknown, because metazoan ORC binds DNA without apparent sequence specificity. Thus, additional factors might be involved in ORC positioning. Our experiments indicate that a family member of the high-mobility ...