PURPOSE The aim of our study was to evaluate the impact of metronidazole (MTZ) on cytotoxicity and DNA synthesis in MCF-7 (estrogen receptor positive) and MDA-MB-231 (estrogen receptor negative) breast cancer cell lines. MATERIAL/METHODS Toxicity of MTZ was determined by MTT test. MCF-7 and MDA-MB-231 cells were incubated with metronidazole used in different concentrations for 24, 48 and 72 h...

We evaluated the antibacterial activities of the crude methanol extract, fractions (I-V) obtained after acid-base extraction and pure compounds from the stem bark of Aspidosperma ramiflorum. The minimum inhibitory concentration (MIC) was determined by the microdilution technique in Mueller-Hinton broth. Inoculates were prepared in this medium from 24-h broth cultures of bacteria (10(7) CFU/mL)....

PURPOSE To evaluate in vitro the effect of nerve growth factor (NGF) on the differentiation of mouse corneal limbal progenitor cells into goblet cells and to observe the expression of mucin-5AC (MUC5AC) mRNA. METHODS Mouse limbal epithelial cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle's medium/Ham's nutrient mixture F12 in vitro, and 63-kDa protein (p63) in cultured cells...

The pharmacokinetics of chloroquine (CQ) and desethylchloroquine (DECQ) were studied in seven male Thai patients with P. vivax malaria and seven healthy male Thais, after the standard oral dosage regimen of CQ (a total dose of 1500 mg given over 3 days). All patients showed a rapid initial response to the treatment with median (range) values of fever and parasite clearance times of 13.7 (2-47) ...

The essential oil of catnip, Nepeta cataria (Lamiacae) was evaluated for behavioral effects on two populations of subterranean termite, Reticulitermes flavipes (Kollar) and R. virginicus (Banks) (Isoptera: Rhinotermitidae). The catnip essential oil contained approximately 36:64 E,Z-nepetalactone and Z,E-nepetalactone, respectively. The time to 50% dissipation (DT50) of the isomers in sand was d...

Two experiments were conducted to evaluate the effects of eCG, temporary 72-h calf removal (CR), or both on dominant follicle (DF) diameter and pregnancy rates (PR) in suckled beef cows. For Exp. 1, we hypothesized that CR, eCG, or both at PGF2α administration concomitant with synchronization of ovulation protocol would increase DF diameter and alter patterns of LH, estradiol (E), and progester...

The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp. in a 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. The effect of submerged ...

This experiment determined whether pulsatile administration of gonadotropin-releasing hormone (GnRH) would induce estrus and ovulation in seasonally anestrous primiparous sows and compared endocrine responses of GnRH-induced sows with those of primiparous sows that exhibited spontaneous estrus after weaning. Seventeen primiparous Landrace X Large White sows farrowed in August 1982, lactated 23....

The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. T...

The aim of the present study was to examine the protective effects and mechanism of sika deer (Cervus nippon Temminck) velvet antler polypeptides (VAPs) against MPP+ exposure in the SH‑SY5Y human neuroblastoma cell line. MPP+ cytotoxicity and the protective effects of VAPs on the SH‑SY5Y cells were determined using an MTT assay. Cell apoptosis and mitochondrial membrane potential were detected ...