The relationship between xenobiotic concentrations in hair and the degree of systemic xenobiotic exposure is poorly defined. The purpose of this study was to evaluate the effect of dose, time, and pigment on the hair incorporation of cocaine (COC) and its metabolites, benzoylecgonine (BE), ecgonine methyl ester (EME), and norcocaine (NCOC). COC was administered by the i.p. route to male Long-Ev...

Recently, LeBeau and Montgomery (1) informed readers that the FBI Laboratory decided to suspend cocaine analysis in hair except children’s hair. This decision was explained by newer research findings, according to which the criteria of the Substance Abuse and Mental Health Services Administration (SAMSHA) for a hair sample positive for cocaine (2) can also be met in contaminated hair specimen (...

The monoclonal antibody (mAb), h2E2, is a humanized version of the chimeric human/murine anti-cocaine mAb 2E2. The recombinant h2E2 protein was produced in vitro from a transfected mammalian cell line and retained high affinity (4 nM Kd) and specificity for cocaine over its inactive metabolites benzoylecgonine (BE) and ecgonine methyl ester. In rats, pharmacokinetic studies of h2E2 (120 mg/kg i...

Cocaine abuse, a growing social problem, is currently estimated from population surveys, consumer interviews and crime statistics. A new approach based on the analysis of cocaine (COC) and metabolites, benzoylecgonine (BE) and ecgonine methyl ester (EME), in water samples was applied to 28 rivers and 37 waste water treatment plants in Belgium using solid-phase extraction and liquid chromatograp...

Cocaine addiction and overdose have long defied specific treatment. To provide a new approach, the high-activity catalytic antibody mAb 15A10 was elicited using a transition-state analog for the hydrolysis of cocaine to nontoxic, nonaddictive products. In a model of cocaine overdose, mAb 15A10 protected rats from cocaine-induced seizures and sudden death in a dose-dependent fashion; a noncataly...

The applications of LC-API-MS in routine forensic toxicological casework were presented. This technique has been used for routine determination of several groups of drugs: opiate agonists (like morphine, codeine, dihydrocodeine and their glucuronides, methadone, buprenorphine) cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester), amphetamine and other psychoactive phenethylam...

Supercritical fluid extraction (SFE) is emerging as a valuable analytical technique for use as an alternative to conventional solid-phase (SPE) and liquid-liquid extraction techniques. It is a relatively new technique based on the use of supercritical fluids for the isolation of analytes from various matrices and is attracting great interest because of the increasing need for a simple, rapid, e...

A series of 2'-substituted cocaine analogs (4-8) was prepared and evaluated in an in vitro dopamine transporter (DAT) binding assay. Compounds 4-7 were prepared by esterifying the 3 beta-hydroxyl group of ecgonine methyl ester (3) using the appropriate acid chloride in the presence of Et3N and benzene. Compound 3 was obtained from cocaine (1) by hydrolysis using 1N HCl to afford ecgonine.HCl wh...

biofuels are the main substitute to fossil fuels. these fuels are less polluting in compari-son to fossil fuels and can be produced from agricultural material residues for use in die-sel engines. in this research work bioethanol was produced from potato waste. it was de-hydrated in a vapor phase using 3a zeolite and was used in combination with sunflower methyl ester oil and diesel fuel blendin...

The quantification of minor alkaloids in seized cocaine can provide information for drug profiling and law enforcement initiatives. This work presents the development validation an analytical method to quantitate samples using gas chromatography with flame ionization detector, after derivatization. was validated accordance ISO/IEC 17.025:2017 requirements. calibration linear (determination coef...