In recent years, a significant amount of sequence data (both genomic and transcriptomic) for Echinococcus spp. has been published, thereby facilitating the analysis of genes expressed during a specific stage or involved in parasite development. To perform a suitable gene expression quantification analysis, the use of validated reference genes is strongly recommended. Thus, the aim of this work ...

BACKGROUND The aim of the present study was to determine the molecular characteristics of Echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using PCR- RFLP of ITS1- rDNA analysis in Golestan Province, northern Iran. METHODS E. granulosus isolates from human patients infected with...

Surgery is still the main treatment in hydatidosis caused by Echinococcus, which is a global health problem in human and animals. So, there is need for some natural protoscolicidal agents for instillation to prevent their reoccurrence at therapeutic doses. In this present investigation, anticestodal activity of one of the endophytic fungi Pestalotiopsis sp. from Neem plant was observed on proto...

Background and objectives: Benzimidazoles such as albendazole are being used in some conditions to treat the hydatid disease, the common parasitic infections of humans and livestock that caused by Echinococcus granulosus. However, treatment with these drugs is not always with complete success. In this study we evaluate the in vitro efficacy of flubendazole (FLBZ) plus praziquantel (PZQ) against...

Objective: We aimed to determine the scolicidal effect of hypochlorous acid and its efficiency at different concentrations. Materials Methods: tested effectiveness in-vitro on sterile gauze concentrations in test tubes. In addition, we compared with activity other agents, povidone-iodine 10% chlorhexidine-gluconate 0.04%. Results: Hypochlorous was 100% effective 5 minutes 1/1 (200 ppm/mL), 1/10...

Cystic echinococcosis (CE) is a cosmopolitan disease caused by the dog tapeworm Echinococcus granulosus. The disease is difficult to diagnose, treat, and control and is responsible for considerable human morbidity and mortality globally. There is an urgent need for new diagnostic tests and new drugs for treatment of CE and the development of a vaccine against adult worms of E. granulosus in dog...

The cestode Echinococcus granulosus, the agent of hydatidosis/echinococcosis, is remarkably well adapted to its definitive host. However, the molecular mechanisms underlying the successful establishment of larval worms (protoscoleces) in the dog duodenum are unknown. With the aim of identifying molecules participating in the E. granulosus-dog cross-talk, we surveyed the transcriptomes of protos...

Cystic echinococcosis is an important parasitic zoonosis caused by the dog tapeworm Echinococcus granulosus. Little is known about adult worm development at the molecular level. Transcription analysis showed that the E. granulosus hormone receptor 3-like (EgHR3) gene was expressed in protoscoleces and adult worms, indicating its role in early adult development. In this study, we cloned and char...