A pot study was used to examine the effects of amendments such as EDTA and elemental sulfur on the growth potential, gas exchange features, uptake and mobilization of Pb by wheat (Triticum aestivum L.) in two texturally different contaminated soils at three levels of EDTA (2, 4, 8 mmol kg(-1) dry soil) and two levels of elemental sulfur (100, 200 mmol kg(-1) dry soil). EDTA resulted in more sol...

To separate both copper and 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid (EDTA) from the waste electroless plating solution by solvent extraction, liquid–liquid equilibria of copper-EDTA complex unbound EDTA ion were experimentally measured. An organic tri-n-octylmethylammonium chloride (TOMACl) was used as solvent. The kerosene TOMACl could extract complexes ions model wastewater. ...

To evaluate and develop methodologies for the extraction of gel-forming extracellular polymeric substances (EPS), EPS from aerobic granular sludge (AGS) was extracted using six different methods (centrifugation, sonication, ethylenediaminetetraacetic acid (EDTA), formamide with sodium hydroxide (NaOH), formaldehyde with NaOH and sodium carbonate (Na2CO3) with heat and constant mixing). AGS was ...

Microwave enhanced chromium extraction from sewage sludge and soil-sludge mixtures was carried out using ethylenediaminetetraacetic acid (EDTA) more biodegradable chelating agents: methyleneglycinediacetic (MGDA), S, S’-ethylenediaminedisuccinic (EDDS) S-carboxyl-L-cysteine (SCLC). Raising the temperature microwave energy had particularly strong effect on Cr efficiency with MGDA all investigate...

By optimizing the raw material ratio for preparation of ethylenediaminetetraacetic acid-type chitosan (EDTA-type chitosan), extraction efficiency rare earth elements was improved. The synthesis with a lower concentration to acetic acid than that in previously reported method confirmed by IR spectra peak assigned as carboxyl group EDTA drastically decreased and amide bond reaction between more f...

We evaluated the inducibility of nitrate-dependent Fe(II)-EDTA oxidation (NDFO) in non-growth, chloramphenicol-amended, resting-cell suspensions of Dechloromonas sp. strain UWNR4 and Acidovorax sp. strain 2AN. Cells previously incubated with Fe(II)-EDTA oxidized ca. 6-fold more Fe(II)-EDTA than cells previously incubated with Fe(III)-EDTA. This is the first report of induction of NDFO by Fe(II).

Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole yeast cells through a rapid and easily scalable procedure that does not require mechanical cell lys...

Constituent availability, or the potentially mobile fraction of the total content, is an important characterization parameter for waste materials and soils. The availability of inorganic species is often evaluated from the results of a sequential extraction procedure at a high liquid-to-solid (LS) ratio using pH control. Several tests maintain pH at 4 and 7 to maximize the solubility of cations...

Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells. To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, r...

Extraction of protease from a local ginger rhizome (Zingiber officinale var. Bentong) was carried out. The effect of extraction pH (6.4, 6.8, 7.0, 7.2, 7.6, 8.0, 8.4, and 8.8) and stabilizers (0.2% ascorbic acid, 0.2% ascorbic acid and 5 mM EDTA, or 10 mM cysteine and 5 mM EDTA) on protease activity during extraction was examined. pH 7.0 potassium phosphate buffer and 10 mM cysteine in combinat...