An erythroid nuclear protein (EF1), originally detected as a protein binding within the nuclease hypersensitive site upstream of the chicken beta H-globin gene, has been purified. This protein of 37,000-39,000 molecular weight binds to three sites within the hypersensitive region: one between the CCAAT and TATA boxes, the second (further upstream) next to a NF1 binding site, and the third adjac...

Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. In this research, an E. faecali...

To develop safer and more effective vectors for gene therapy of X-linked severe combined immunodeficiency (SCID-X1), we have evaluated new self-inactivating lentiviral vectors based on the HIV virus. The CL20i4-h c-Revgen vector contains the entire human common chain ( c) genomic sequence driven by the c promoter. The CL20i4-EF1 -h cOPT vector uses a promoter fragment from the eukaryotic elonga...

Despite advances, identification and formulation of safe and effective vaccine for long-lasting protection against leishmaniasis is still inadequate. In this study, we have identified a novel antigen, leishmanial elongation factor-1α (EF1-α), as an immunodominant component of solubilized leishmanial membrane antigens that reacts with visceral leishmaniasis (VL) sera and induces cellular prolife...

An innovative biological energy has been observed related to the eye sight and fusing with another energy over tap water through a small metallic chain. The fused energy has been repeatedly described under standard conditions and it is characteristics vary according to the condition of the eye sight and the water content. This energy may be called: "EFI" (energy functioning, by fusion, independ...

Aspartylation of mammalian tRNAAsp by bacteria-expressed human aspartyl-tRNA synthetase (hDRS) was examined. The kinetics of the aspartylation of tRNA was consistent with the following reaction pathway, [formula: see text] where E, represents aspartyl-tRNA synthetase. A set of rate constants was obtained which fit single turnover time courses at varying concentrations of the enzyme, tRNA, and A...

The protein kinase inhibitor H7 [1-5(isoquinolinesulfonyl-2-methylpiperazine)] together with a temperature shift to 42 degrees C was found to reproducibly and efficiently induce differentiation of avian erythroblasts transformed with the avian erythroblastosis virus containing v-erbA and a temperature-sensitive v-erbB oncogene. Although a temperature shift to 42 degrees C without H7 results in ...

CaBP4 modulates Ca(2+)-dependent activity of L-type voltage-gated Ca(2+) channels (Cav1.4) in retinal photoreceptor cells. Mg(2+) binds to the first and third EF-hands (EF1 and EF3), and Ca(2+) binds to EF1, EF3, and EF4 of CaBP4. Here we present NMR structures of CaBP4 in both Mg(2+)-bound and Ca(2+)-bound states and model the CaBP4 structural interaction with Cav1.4. CaBP4 contains an unstruc...

Using the method of gene targeting in mouse embryonic stem cells, regulatory function of d EF1, a zinc finger and homeodomain-containing transcription factor, was investigated in vivo by generating the d EF1 mutant mice. The mutated allele of d EF1 produced a truncated form of the d EF1 protein lacking a zinc finger cluster proximal to COOH terminus. The homozygous d EF1 mutant mice had poorly ...

Urf a is located between the genes encoding subunits 6 and 8 and strain 50 as reference (B.F.Lang, pers. comm.). Strain EF1 of ATPase (qtp6, atp8) and flanked by the genes for tRNA^" and versus 50 shows four amino acid changes (GAA —GAT:E—D; tRNA'' {tmq, tmi). This urf seems unique for S. pombe. Here CTT-CCT:L—P; ATT-GTT:I -V; ATA—GTA:I—V) and we present the urf a sequence of 2 5. pombe strains...