Background: DNA amplification method has been developed for identifying and discriminating Salmonella serovars, using specific primers at the genus and serovar levels and to identify the S. Enteritidis, S. Dublin, S. Gallinarum and S. Pullorum. Objectives: This study was conducted for molecular identification and discrimination among some important Salmonella serovars. Methods: Fifty isolates o...

conclusions the b3, b5, b7, b8 and b9 subgenotypes can be clustered into quasi-sub-genotype b3 and the open reading frame of hbv has a start codon preference; however, whether a mutation in the start codon in the pre-s2 region has an impact on survival and replication of hbv remains to be determined. background the number of cases of hbv infection reported by the who for each district and count...

Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for the identification of regulatory sequences in genomes. We tested this approach to identify regulatory sequences that control expression in the midline of the zebrafish embryo. We first isolated a s...

We have established a cartilaginous fish cell line [Squalus acanthias embryo cell line (SAE)], a mesenchymal stem cell line derived from the embryo of an elasmobranch, the spiny dogfish shark S. acanthias. Elasmobranchs (sharks and rays) first appeared >400 million years ago, and existing species provide useful models for comparative vertebrate cell biology, physiology, and genomics. Comparativ...

The suspensor is a terminally differentiated embryonic region that connects the embryo to surrounding tissues during early seed development. Most seed-bearing plant embryos contain suspensor regions, which occur in a wide variety of sizes and shapes, and suspensor-like structures are present in the embryos of some lower land plants. Recent technological advances, including novel genomics approa...

The VEGF receptor, FLK1, is essential for differentiation of the endothelial lineage and for embryonic vascular development. Using comparative genomics, we have identified conserved ETS and Krüppel-like factor (KLF) binding sites within the Flk1 enhancer. In transgenic studies, mutation of either site results in dramatic reduction of Flk1 reporter expression. Overexpression of KLF2 or the ETS t...

Buffalo holds the unique potentiality among the livestock sector at tropical and subtropical area. The faster multiplication of superior genotypes and the conservation of endangered buffalo breeds are the important issue. Recent advances in assisted reproductive technologies (ART), including estrus/ovulation synchronization, multiple ovulation and embryo transfer (MOET), in vitro embryo product...

Coconut palm (Cocos nucifera) is a symbol of the tropics and a source of numerous edible and nonedible products of economic value. Despite its nutritional and industrial significance, coconut remains under-represented in public repositories for genomic and transcriptomic data. We report de novo transcript assembly from RNA-seq data and analysis of gene expression in seed tissues (embryo and end...

Single-embryo transfer is becoming increasingly common in in vitro fertilization (IVF) treatment as a means of reducing multiple pregnancy rates leading to a higher incidence of medical, perinatal and neonatal complications. Consequently, selecting embryos with the highest implantation potential is of great importance in assisted reproductive technology. To date, the choice of the best embryos ...