Background: Activation of bone morphogenetic protein 4 (BMP4) signaling pathway in embryonic stem (ES) cells plays an important role in controlling cell proliferation, differentiation, and apoptosis. Adverse effects of BMP4 occur in a time dependent manner; however, little is known about the effect of different time exposure of this growth factor on cell number in culture media. In this study, ...

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...

Background: Transgenic mice are being considered as invaluable tool in biological sciences towards comprehension of the cause of the genetic diseases. Manipulated embryonic stem (ES) cells are used to produce loss-of-function mutant mice. Microinjection of manipulated ES cells into blastocoel cavity, and morula fusion are the two main techniques in producing transgenic mice. So...

background: mesenchymal stem cells (mscs) are important candidates for msc-based cellular therapy. current paradigm states that mscs support local progenitor cells in damaged tissue through paracrine signaling. therefore, study of paracrine effects and secretome of mscs could lead to the appreciation of mechanisms and molecules associated with the therapeutic effects of these cells. this study ...

regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. human embryonic stem cells (hescs) hold two important properties called self renewal and pluripotency. however, the use of ...

objective: mir-302-367 is a cluster of polycistronic micrornas that are exclusively expressed in embryonic stem (es) cells. the mir-302-367 promoter is functional during embryonic development but is turned off in later stages. motivated by the cancer stem cell hypothesis, we explored the potential expression of mir-302 in brain tumor cell lines. materials and methods: in the present experimenta...

Background This research studies the effects of activation and inhibition of Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem (ES) cell-like cells. MaterialsAndMethods To carry on this experimental study, the effects of activation and inhibition of Wnt3A signaling in buffalo ES cell-like cells were examined using Bio (0.5 mM) combined with WNT3A (200 ng/ml), as an activator, ...

the stem cells can be considered as the miracle of creation, because, they are a major reservoir of the bioactive factors, molecules and cells. these reservoir cells play a major role in development and growth of the tissues and organs. regardless of the differences in differentiation capacity of embryonic and adult stem cells, both the cells are rich sources of stem cells with therapeutic appl...

backgrounds: a straight-forward way to identify whether a drug or environmental chemical can be harmful to unborn baby is to evaluate its effect on laboratory animals. all invivo methods need large number of animal and are therefore time consuming and expensive. however, the thousands of chemicals in need of testing, to reduce the spending of live animals, an assortment of in vitro assays has b...