The kinetics of triplex folding/unfolding is investigated by the single-molecule fluorescence resonance energy transfer (FRET) technique. In neutral pH conditions, the average dwell times in both high-FRET (folded) and low-FRET (unfolded) states are comparable, meaning that the triplex is marginally stable. The dwell-time distributions are qualitatively different: while the dwell-time distribut...

Photonic wires were constructed by sequentially arranging up to 7 fluorophores along a concatenated DNA scaffold. This yielded nanostructures displaying from one- to six-energy transfer steps where end-to-end efficiency reflected the multiple underlying photophysical processes and the ability of long-range interactions to compensate for localized non-ideal dye behaviour.

Graphene oxide can act as an ultrahighly efficient quencher for upconversion nanophosphors and thus, an extraordinarily sensitive biosensing platform is constructed.

We successfully prepared organic core/diffuse-shell nanorods, which presents fluorescence resonance energy transfer from the core to shell components.

Caught in the act: The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high back...

Single-molecule studies can be used to study biological processes directly and in real-time. In particular, the fluorescence energy transfer between reporter dye molecules attached to specific sites on macromolecular complexes can be used to infer distance information. When several measurements are combined, the information can be used to determine the position and conformation of certain domai...

Cameleons are genetically encoded fluorescence resonance energy transfer (FRET)-based Ca(2+) indicators. Attempts to use cameleons to detect neural activity in vertebrate systems have been largely frustrated by the small FRET signal, in contradistinction to the higher signals seen in Drosophila and Caenorhabditis elegans. We have developed a statistical optimization method capable of detecting ...

Tunable emission of binary organic nanobelts was realized via the fluorescence resonance energy transfer (FRET) process, which can be exploited for the detection of acid and basic chemical vapors.

Single molecule fluorescence resonance energy transfer has been extensively used to measure distance changes and kinetics in various biomolecular systems. However, due to complications involving multiple de-excitation pathways of the dyes, the absolute inter-dye distance information has seldom been recovered. To circumvent this we directly probe the relative variations in the quantum yield of i...

Förster resonance energy transfer (FRET) has been used to show that the average distance between proteins decreases when the protein is immobilized within polymer scaffolds, indicating that the immobilization process is inducing aggregation.