Implantation support medium transfer (ISME-T) is referred as cultivation and transfer of embryos using advanced enriched medium that all nutrients and energy sources needed for an optimal embryo development. They may serve as cultivation as well as transferring medium. Enriched cultivation medium reduces the embryo stress through in vitro fertilization (IVF) process by providing a more physiolo...

Fig 6. Effect of Zn -enrichment on claudin-1 displacement rate. (A) NP-40-soluble and (B) NP-40insoluble fractions. Gray: calcium-free medium, black: Zn-enriched medium, white: Zn-enriched medium treated with STb for 6, 12 and 24 h. Lower panel: Immunoblot showing claudin-1 and GAPDH used to evaluate their relative amounts. NP-40 cell extracted proteins were separated on a 12% acrylamide SDSPAG...

The thiol-disulfide oxidoreductase DsbA carries out oxidative folding of extra-cytoplasmic proteins by catalyzing the formation of intramolecular disulfide bonds. It has an important role in various cellular functions, including cell division. The purple non-sulfur bacterium Rhodobacter capsulatus mutants lacking DsbA show severe temperature-sensitive and medium-dependent respiratory growth def...

A sugar fermentation medium for the confirmation of Neisseria and related species was developed. The medium contained a commercial supplement and a hemoglobin source prepared from lysed sheep erythrocytes. Bicarbonate in the medium substituted for a CO2-supplemented atmosphere. The medium was dispensed into screw-capped tubes. This medium was compared to cystine-Trypticase agar and the modified...

currently, white fish or kutum rutilus frisii is considered to be a good candidate for commercial aquaculture. however, little information is available regarding the nutritional requirement of this fish fry. experiments were conducted to examine the effects daphnia magna as live food cultured on two freshwater green algae, chlorella sp. and scenedesmus obliquus enriched with different b group v...

A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, such as AC broth (Difco), the activity of the mutant ligase increased 10- to 20-...