Detection and Frequency of Enterotoxin (cpa,cpe) Genes Clostridium perfringens Isolated from Dehydrated Vegetables by PCR

yersinia enterocolitica is newly included in the classification of both the invasive bacteria, e.g. shigella, and the toxicogenic bacteria, e.g. escherichia coli. five human strains and 45 environmental strains were used to produce y.enterocolitical enterotoxin at 40c, 240c and 370c using infant mouse assay. at 250c enterotoxin was produced by 10 environmental strains and by all of the 3 human ...

elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

Polymyxin B-induced release of enterotoxin from Escherichia coli strain H-10407 was demonstrated. Incubation of E. coli cells derived from 6-h cultures with polymyxin caused the rapid release of enterotoxin with a molecular weight of approximately 20,000, as estimated by the gel filtration technique. The rapidity of the release of enterotoxin indicates that it probably resides in the periplasmi...

Natural cholera toxoid appears to act as a competitive inhibitor of cholera enterotoxin and is thus a useful tool for studying the interaction of cholera enterotoxin with cell membranes. Cholera enterotoxin binds to gut mucosa more rapidly than does its natural toxoid. Once binding occurs, however, it appears to be prolonged for both materials. Formalinized cholera toxoid has no inhibitory effe...

Two peptide bonds of staphylococcal enterotoxin C, were hydrolyzed concurrently at quite different rates during limited digestion with trypsin. A Lys-Val at about position 92 in the disulfide loop was the first bond cleaved, followed by a Lys-Asx at about position 57 on the NH2-terminal side of the loop. Preparations of singly cleaved material (enterotoxin C1-T1) contained about 93% of the clea...

staphylococcus aureus is a major human pathogen producing different types of toxins. enterotoxin a ( sea ) is the most common type among clinical and food-related strains. the aim of the present study was to examine the prevalence of sea in clinical isolates of s. aureus . moreover, the correlation between sea producing strains and type of infection as well as resistance to antibiotics is also ...

objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...

Enterotoxin of Clostridium perfringens was assayed and compared with toxicity in mice and erythemal activity in guinea pigs. Conversion factors were used to express these biological activities of crude enterotoxin in terms of weight of pure enterotoxin protein. One microgram of enterotoxin was equivalent to 3.41 erythema units and to 0.68 mouse median lethal dose.