Background and Aims: Staphylococcus aureus (S. aureus) is considered as one of the most dangerous pathogenic bacteria due to the production of extracellular toxins. The objective of this study was to determine the prevalence of S. aureus and to characterize the recovered strains for their enterotoxin-producing genes in raw cow milk. Materials and Methods: During 9 months duration of the s...

ABSTRACT: The aim of this study was to molecularly identify different species Aeromonas isolated from farmed tambaqui (Colossoma macropomum) North Brazil, and evaluate their pathogenic potential by the presence virulence genes. From extraction bacterial DNA, PCR (polymerase chain reaction) primers 16S rDNA, aerA (cytolytic enterotoxin), ast (cytotoxic enterotoxin) act were performed. Of 24 isol...

background and objectives:   staphylococcus aureus is one of the main causatives of bovine mastitis. resistance of some strains to methicillin, can complicate the treatment of its infections. on the other hand, enterotoxin production is also im- portant. therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in s. aureus isolates caused bovine m...

Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the nine Staphylococcal enterotoxins (SEs) and enterotoxin gene profiles in S. aureus isolates derived from raw bovine milk....

The genes for a new enterotoxin were cloned from Escherichia coli SA53. The new toxin was heat labile and activated adenylate cyclase but was not neutralized by antisera against cholera toxin or E. coli heat-labile enterotoxin. Subcloning and minicell experiments indicated that the toxin is composed of two polypeptide subunits that are encoded by two genes. The two toxin subunits exhibited mobi...

Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...

conclusions these findings illustrated the presence and persistence of clonal groups of mrsa strains, in chicken meat in isfahan, iran, that serve as reservoirs to disseminate bacteriophage encoded enterotoxin and virulence agents. results all 116 isolated mrsa strains were discriminated into seven php types consisting of seven common types (cts) and were positive for meca gene. all isolates ha...

milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. conversely, it can be a vehicle for several pathogenic bacteria such as staphylococcus aureus. this study aimed to analyze the frequency of genes encoding the nine staphylococcal enterotoxins (ses) and enterotoxin gene profiles in s. aureus isolates derived from raw bovine milk....

The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulas...

AIMS   The aim of this study was to determine whether endophytic Bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (Nhe) and haemolysin BL (HBL) and, therefore, have the potential to cause diarrhoeal illness in humans. METHODS AND RESULTS   PCR followed by sequencing confirmed the presence of enterotoxin genes nheA, nheB, nheC, hblA, hblC, hblD in...