Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feed...

Banana besides its nutritive value the biomass can be used as resources for natural fibre. North east India and Assam in particular has a vast potential of extraction and value addition of natural fibre, especially from banana. Banana farming generates huge quantities of biomass most of which goes as waste due to non‐availability of suitable technology for its commercial utilization. Banana fib...

In the present study, we investigated the ability of an immobilized phospholipase A1 (PLA1) in degumming of soybean oil. The enzyme was immobilized by simple adsorption on bentonite without any further modification. The free and immobilized PLA1 were characterized by Fourier Transform InfraRed (FT-IR) spectroscopy and X-Ray Diffraction (XRD). The immobilizat...

Abelmoschus manihot (L.) Medic has drawn much attention recently due to its potential beneficial health effects. The roots, flowers and seeds of A.manihot play an important role as medical materials. In this paper, the result of the chemical composition testing shows that the main constituent of A.manihot bast of the stems is cellulose (41.80 %). A.manihot fibers are extracted from A.manihot ba...

This study compared the profiles of fatty acids, phytosterols, and polyamine conjugates in conventional commercial corn oil extracted from corn germ and in two ‘‘new-generation’’ corn oils: hexane-extracted corn fiber oil and ethanol-extracted corn kernel oil. The fatty acid compositions of all three corn oils were very similar and were unaffected by degumming, refining, bleaching, and deodoriz...

BACKGROUND The conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. Pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption. Pectate lyase PEL168 from Bacillus subtilis has been previously characterized and the protein structure was resolved. Ho...

Enzymes are the catalysts of all reactions in living systems. These reactions are catalysed in the active sites of globular proteins. The proteins are composed by amino acids with a variety of side chains ranging from non-polar aliphatic and aromatic to acidic, basic and neutral polar. This fact allows to a globular 3D protein to create in the active site all ranges of microenvironments for cat...