Now, more than ever, enzymology and its development can be considered of vital importance to the progression of the biological sciences. With an increase in the numbers of enzymes being identified from genomic studies, enzymology is key to defining the structural and functional properties of these enzymes in order to establish their mechanisms of action and how they fit into metabolic networks....

One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance the catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes a single C-H-C transfer, is used as a model system to address this question. Experimental and computational studies have proposed a dynamic network that includes two residue...

organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...

Enzymes are remarkable catalysts that lie at the heart of biology, accelerating chemical reactions to an astounding extent with extraordinary specificity. Enormous progress in understanding the chemical basis of enzymatic transformations and the basic mechanisms underlying rate enhancements over the past decades is apparent. Nevertheless, it has been difficult to achieve a quantitative understa...

Life as we know it heavily relies on biological catalysis, in fact, in a very nonromantic version of it, life could be considered as a series of chemical reactions, regulated by the guarding principles of thermodynamics. In ancient times, a beating heart was a good sign of vitality, however, to me, it is actually the presence of active enzymes that counts… Though we do not usually pay attention...

considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. in enzymatic digestion method extracted teeth as denta...

Considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. Meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. In enzymatic digestion method extracted teeth as denta...