Recent advances in single-molecule enzymology are reviewed. The theoretical underpinning of sincle-molecule enzymatic behaviors is discussed and exemplified by experiements and statistical analyses. In particular, the manifestations of the Michaelis-Menten mechanism, the kinetic scheme with sequential intermediates, and dynamic disorder in single molecule data are presented. A survey of current...

The increasing use of enzymes as aids to clinical diagnosis has made it mandatory for clinical biochemists to develop techniques and instrumentation to enable large numbers of specimens to be processed quickly with adequate accuracy. The main objective in enzyme assay is to determine the initial reaction rate, as it is only during this period that there is a linear relationship between the rate...

1 . Introduction 2. What is Automation? 3. Solution-Based, Absorption Spectrophotometric Methods 3.1. Specimen Identification 3.2. Specimen Preparation 3.3. Specimen Integrity 3.4. Pipeting Specimens 3.5. Temperature Control 3.6. Absorbance Accuracy 3.7. Wavelength Accuracy 3.8. Time Control 3.9. Cuvet Control 3.10. Control of Interferences 3.10.1. Keto Acids 3.10.2. Ammonium Ions 3.10.3. Glyce...

In a sequence of coupled enzyme reactions the steady-state production of product is preceded by a lag period or transition time during which the intermediates of the sequence are accumulating. Provided that a steady state is eventually reached, the magnitude of this lag may be calculated, even when the differentiation equations describing the process have no analytical solution. The calculation...

A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.

*Corresponding author: Dominika Szőke, UOC Patologia Clinica, Azienda Ospedaliera “Luigi Sacco”, Via GB Grassi 74, 20157 Milan, Italy, Phone: +39 02 39042683, Fax: +39 02 39042364, E-mail: [email protected]; and Clinical Pathology Unit, “Luigi Sacco” University Hospital, Milan, Italy Assunta Carnevale and Sara Pasqualetti: Clinical Pathology Unit, “Luigi Sacco” University Hospital, Milan...