Back Cover: In article number 2100121, Suchetan Pal and co-workers develop a family of multi-responsive polymer hydrogels crosslinked with bis boronic acid molecules. Hydrogels tetrahydroxydiboron show glucoseresponsive degradation in the presence glucose oxidase (GOX) enzyme. Furthermore, insulin releases under high conditions physiological milieu.

An intracellular glucose oxidase was isolated from the mycelium extract of a locally isolated strain of Aspergillus niger UAF-1. The enzyme was purified to a yield of 28.43% and specific activity of 135 U mg(-1) through ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The enzyme showed high affinity for D-glucose with a Km value of 2.56 mM. The enzyme exhibited ...

Developing countries have a high prevalence of diabetes and their populations are suffering from associated adverse factors. Such a frequency requires more effective diagnosis, mostly achieved by glucose diagnostic kits. Although high priced kits are available in market but local production of such kits can be highly cost effective and may confer the decline in incidence of the disease. Glucose...

Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) result...

Enzyme-based biofuel cells (EBFCs) are systems that use a variety of organic compounds to produce electricity through oxido-reductase enzymes, such as oxidase or dehydrogenase as biocatalysts immobilized on electrodes. In this study, a single-chamber EBFC consisting of carbon electrodes that operating at ambient temperature in phosphate buffer, pH 7 is reported. The EBFC anode was based on gluc...

Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated impact temperature pulses on GOx that was drop-coated flattened Pt microwires. The wires were heated by alternating current. sensitivity towards and stability amperometry. An up to 22-fold increase observed. Spatially resolve...

Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzym...

BACKGROUND Continuous amperometric sensors that measure glucose or lactate require a stable sensitivity, and glutaraldehyde crosslinking has been used widely to avoid enzyme loss. Nonetheless, little data is published on the effectiveness of enzyme immobilization with glutaraldehyde. METHODS A combination of electrochemical testing and spectrophotometric assays was used to study the relations...

This laboratory seeks to quantitatively determine glucose concentration in common beverages. The method employed is enzyme catalyzed oxidation of glucose, quantified by chronoamperometry. With an inexpensive patterned electrode and low cost glucose oxidase enzyme, an electrochemical sensor is constructed to selectively measure glucose in complicated beverages which can present as analytically c...

The thermostabilization of some enzymes by amidination with dimethylsuberimidate was surveyed. In the enzymes investigated, a moderate increase of thermostability in glucose oxidase and invertase, and prominent increase in lactate dehydrogenase, alcohol dehydrogenase and ascorbate oxidase were observed. The increase of thermostability by amidination was suggesting the synergism of the enhanceme...