An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

Sandwich enzyme-linked immunosorbent assay (ELISA) microarrays can simultaneously quantify the levels of multiple diagnostic targets in a biological sample. However, as with traditional ELISA diagnostics, endogenous antibodies in patient sera can cause interference. We demonstrate here that reducing the diagnostic capture antibody to its minimal functional unit (i.e., a single-chain antibody fr...

Biotin (vitamin B7) is a water-soluble vitamin used as co-enzyme for carboxylases essential human metabolism. The high affinity to streptavidin makes biotin an important substance in immunoassays. Excessive intake due over-the counter supplements has become problematic because of the effects on laboratory test results. There have been no reports biotin-induced thyroid immunoassay interference K...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

CONTEXT Ashwagandha, a popular Ayurvedic medicine, is now available in the United States. Alkaloids found in this herb have structural similarity with digoxin. OBJECTIVE To study potential interference of Ashwagandha with serum digoxin measurement by immunoassays. Potential interference was also investigated with immunoassays for 11 other commonly monitored drugs. In addition, interaction of ...

The present work reports the development and application of an amperometric biosensor based on carbon paste electrode modified with laccase enzyme, glutaraldehyde, gold nanoparticles (Lac-Glu-AuNPs/CPE) for determination neurotransmitter dopamine (DA). materials were characterized morphologically chemically using scanning electron microscopy (SEM), transmission (TEM), cyclic voltammetry. Optimi...