Grapevine powdery mildew is one of the most damaging fungal diseases. Therefore, a precise understanding of the grapevine disease resistance system becomes a subject of significant importance. Plant microRNAs(miRNAs) have been implicated to play regulatory roles in plant biotic stress responses. In this study, high-throughput sequencing and miRDeep-P were employed to identify miRNAs in Chinese ...

Plant resistance proteins mediate pathogen recognition and activate innate immune responses to restrict pathogen proliferation. One common feature of these proteins is an NB-ARC domain. In this study, we characterized a gene encoding a protein with an NB-ARC domain from wild Chinese grapevine Vitis pseudoreticulata accession "Baihe-35-1," which was identified in a transcriptome analysis of the ...

Disease resistance gene pyramiding is a widely used strategy to enhance durability. Marker-assisted selection (MAS) was applied pyramide the alleles Run1 and Ren3, which confer against grape powdery mildew (Erysiphe necator). Two F1 full-sibs carrying Ren3 in heterozygosity were selfed develop breeding populations analysis. From 637 genotyped plants, 313 (50.6%) had pyramided. Seven (1.1%) of t...

RNA expression profiling of obligately parasitic plant microbes is hampered by the requisite interaction of host and parasite. This can be especially problematic in the case of powdery mildews, such as Erysiphe necator (syn. Uncinula necator), which grow superficially but tightly adhere to the plant epidermis. We developed and refined a simple and efficient technique in which nail polish was us...

Powdery mildew, caused by the fungus Erysiphe (Uncinula) necator is a grapevine disease of nearly universal importance, often requiring 10 to 15 annual fungicide applications for its control. In the field, disease severity on fruit and leaves provided good sunlight exposure was typically twoand sevenfold lower, respectively, than on those in the shade. Controlled-environment studies showed that...