A sequential injection system for the determination of nitrate (NO3 ) in vegetables was developed to automate this determination, allowing for substantially reduced reagent consumption and generated waste using low-cost equipment. After extraction with water and filtration, the extracted nitrate is reduced inline to nitrite in a copperized cadmium (Cd) column and determined as nitrite. Accordin...

Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of Ethacridine lactate in pharmaceutical formulations. Method A is based on the oxidative coupling of Eathacridine Lactate with 3-methyl-2-benzothiazolinone hydrazone in the presence of Fe(III) to form a violet coloured chromoscme with a absorption maximum of 535nm. Method B is based on...

Antimicrobials are widely used in topical formulations as preservatives or as therapeutically active agents. Photosensitization by such compounds has not yet been studied systematically. To identify possible phototoxic properties, antimicrobials (benzyl alcohol, bronopol, chloracetamide, clioquinol, diazolidinyl urea, ethylenediamine dihydrochloride, formaldehyde, glutaraldehyde, imidazolidinyl...

Depletion of intracellular zinc by N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) induces p53-mediated protein synthesis-dependent apoptosis of mouse cortical neurons. Here, we examined the requirement for poly(ADP-ribose) polymerase (PARP)-1 as an upstream regulator of p53 in zinc depletion-induced neuronal apoptosis. First, we found that chemical inhibition or genetic deletion of ...

A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-colored product absorbing at 496 nm. Nitric oxide levels were determined in culture media from th...

Background: nitrogen oxides occur in the atmosphere in Six forms of namely: N2O , NO , NO2 , N2O3, N2O4 and N2O5 .Among these oxides NO2 is the most important air pollutant because of its relatively high toxicity and its ubiquity in ambient air. In this research Griess-Saltzman method was utilized for determination of nitric oxide and nitrogen dioxide in the atmosphere of different laboratori...