An efficient protocol of common bean transformation using Agrobacterium tumefaciens is still unavailable. Several regeneration reports are available using cotyledonary node or embryonic axis as the starting explants. These explants were used for direct organogenesis exploiting the meristems for direct shoot production. Attempts were made to transform cotyledonary node explants and embryonic axe...

PURPOSE To determine whether resurfacing submacular human Bruch's membrane with a cell-deposited extracellular matrix (ECM) improves retinal pigment epithelial (RPE) survival. METHODS Bovine corneal endothelial (BCE) cells were seeded onto the inner collagenous layer of submacular Bruch's membrane explants of human donor eyes to allow ECM deposition. Control explants from fellow eyes were cul...

Explants of leaves, petals, anthers, styles and ovaries of C. speciosus, C. cancellatus, C. gilanicus and C. almehensis were cultured on LS media with three different combinations of NAA and BAP in the dark. Callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. Corm explants from these species were also cultured on...

The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status plants are old and showing diseased attacked. To support rejuvenation, provision seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed study effect 2,4-D, BA TDZ on callus formation embryo induction from leaf explants. Explan...

Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...

PURPOSE This study investigates the effects of heat shock treatment and the role of Hsp70 and Hsp90 on tranforming growth factor beta 2 (TGF-beta2)-induced epithelial-to-mesenchymal transition (EMT) in rat lens epithelial explants. METHODS Rat lens epithelial explants from 7 to 10 day-old Wistar rats were dissected and incubated for 24 h before treatment. The explants were divided into eight ...

Rumex thyrsiflorus Fingerh. is one of the few dioecious plant species, which have sex chromosomes. We conducted the preliminary experiments to determine the type of morphogenesis of R. thyrsiflorus explants cultured in vitro and to verify, using PCR-based methods, if there is the relationship between sex and morphogenetic response of explants micropropagated under in vitro conditions. The resul...

In order to enhance the system of potato transformation and further regeneration, four potato cultivars were transformed using the Agrobacterium tumefaciens harboring â-glucuronidase (GUS) gene.The results revealed that the highest percentages of shoot formation obtained were for Spunta cv. Stem explants resulted in more callus and regeneration after transformation and selection than leaf expla...