Chinese hamster ovary (CHO-K1) cells were pulse labeled with [3H]serine, and the synthesis of phosphatidyl[3H]ethanolamine from phosphatidyl[3H]serine during the subsequent chase was used as a measure of lipid translocation to the mitochondria. When the CHO-K1 cells were pulse labeled and subsequently permeabilized with 50 micrograms of saponin per ml, there was no significant turnover of nasce...

Assisted reproductive techniques (ART) have now been extensively incorporated in the management of infertile couples. But even after rapid methodological and technological advances the success rates of these procedures have been below expectations. This has led to development of many sperm preparation protocols to obtain an ideal semen sample for artificial reproduction. Sperm apoptosis has bee...

In the plasma of patients with acute myocardial infarction the values for phosphatidyl ethanolamine are on the average 50% to 100% higher than in patients with chronic coronary heart disease and up to 400% higher than in normal individuals. Those for phosphatidyl serine are about 50% higher than in patients with chronic coronary heart disease and up to 300% higher than in normal individuals. Ex...

Phospholipids such as phosphatidyl inositol and phosphatidyl serine inhibit the binding of R5020 and progestin receptors. The effect of phospholipids on the binding of estrogen and estrogen receptors of rat uterine cytosol was studied. Phosphatidyl choline, sphingomyelin, phosphatidyl inositol, phosphatidyl ethanolamine, cardiolipin, and phosphatidic acid inhibited the binding of estradiol and ...

Phospholipids are part of the milk fat globule membrane. Functional and technological properties make them interesting for the development of functional foods or as ingredients of technological interest. In this study an analytical procedure for determination of major phospholipids in milk (phosphatidyl Ethanolamine, phosphatidylinositol, phosphatidyl Choline, phosphatidyl Serine and sphingomye...

Background: Sperm that bypasses natural apoptosis may find their way into semen. In order to avoid the insemination of such sperm during an intracytoplasmic sperm injection (ICSI) treatment, novel sperm selection procedures such as the Zeta procedure have been implemented. Therefore, the aim of this study was to evaluate whether this procedure, in addition to reducing the number of immature or ...

This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis. It is based on the principle that normal cells are hydrophobic in nature as they express phosphatidyl serine in the inner membrane (side facing the cytoplasm) and when the cells...

In the preceding paper (1) the synthesis of 0-(distearoyl L-ac-glycerylphosphoryl) L-serine (I) and its use in the elucidation of the structure and configuration of ox brain phosphatidyl serine have been reported. While trying to establish the identity of the reduction product of the natural phosphatidyl serine and synthetic n-a-(distearoyl)phosphatidyl L-serine by comparing their specific rota...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...