Single-channel properties of the Xenopus inositol trisphosphate receptor (IP3R) ion channel were examined by patch clamp electrophysiology of the outer nuclear membrane of isolated oocyte nuclei. With 140 mM K+ as the charge carrier (cytoplasmic [IP3] = 10 microM, free [Ca2+] = 200 nM), the IP3R exhibited four and possibly five conductance states. The conductance of the most-frequently observed...

An immortal nonhormone-producing cell line with a characteristic star-shaped morphology, named Tpit/F1, was derived from an anterior pituitary gland of a temperature-sensitive large T antigen transgenic mouse. To characterize Tpit/F1 cells, we performed cytological studies, which revealed that Tpit/F1 cells express the messenger RNAs of neruonal nitric oxide (NO) synthase, S-100 protein, basic ...

this study seeks to examine how a company can select the best intermediary for its marketing channels with minimum of criteria and time. a theoretical framework is proposed based on the most important tasks of intermediary and the criteria to measure them. there are four basic tasks and thirty criteria in three independent levels. subsequently, an exploratory case study in iranian food industry...

We introduce various exchange interactions in a model Hamiltonian for Ce3+ ions in cubic symmetry with three configurations (f0,f1,f2). With the impurity pseudo spin SI = 1/2, our Hamiltonian includes: (i) One-channel Sc = 1/2 Anderson model; (ii) Two-channel Sc = 1/2 Anderson model; (iii) An unforseen one-channel Sc = 3/2 Anderson model with a non-trivial fixed point; (iv) Mixing exchange inte...

Mitochondria maintain tight regulation of inner mitochondrial membrane (IMM) permeability to sustain ATP production. Stressful events cause cellular calcium (Ca(2+)) dysregulation followed by rapid loss of IMM potential known as permeability transition (PT), which produces osmotic shifts, metabolic dysfunction, and cell death. The molecular identity of the mitochondrial PT pore (mPTP) was previ...

Construction of the bacterial flagellum in the cell exterior proceeds at its distal end by highly ordered self-assembly of many different component proteins, which are selectively exported through the central channel of the growing flagellum by the flagellar type III export apparatus. FliI is the ATPase of the export apparatus that drives the export process. Here we report the 2.4 A resolution ...

During auditory stimulation with several frequency components, distortion products (DPs) are generated as byproduct of nonlinear cochlear amplification. After generated, DP energy is reemitted into the ear channel where it can be measured as DP otoacoustic emission (DPOAE), and it also induces an excitatory response at cochlear places related to the DP frequencies. We measured responses of 91 i...

Unrehearsed spoken language often contains disfluencies. In order to correctly interpret a spoken utterance, any such disfluencies must be identified and removed or otherwise dealt with. Operating on transcripts of speech which contain disfluencies, we study the effect of language model and loss function on the performance of a linear reranker that rescores the 25-best output of a noisychannel ...

the aim of the present study was to examine the effect of grafting of watermelon hybrids ‘obla f1’ and ‘vanessa f1’ on to cucurbita maxima × cucurbita moschata rootstock tz 148 and lagenaria sp. rootstock ‘dias f1’, on the volatiles and yield of fruit and the plant growth. fruit volatiles analysis showed the presence of two aldehydes, namely (e)-2-nonenal and (e,z)-2,6-nonadien-1-al, with (e)-2...

Optimal design of wireless systems in the presence of fading involves the instantaneous allocation of resources such as power and frequency with the ultimate goal of maximizing long term system properties such as ergodic capacities and average power consumptions. This yields a distinctive problem structure where long term average variables are determined by the expectation of a not necessarily ...