The activity of the NhaA Na+/H+ antiporter of Vibrio parahaemolyticus is inhibited by amiloride. We found an amino acid sequence in the NhaA that was identical to a putative amiloride binding domain of the Na+/H+ exchanger in mammalian cells. We constructed mutant NhaAs that had amino acid substitutions in the putative amiloride binding domain by site-directed mutagenesis. These include V62L (V...

An outbreak of extended spectrum â lactamase producing Klebsiella pneumoniae (ESBLKp) in a neonatal unit was controlled using simple measures. Normally, the control of such infections can be time consuming and expensive. Seven cases of septicaemia resulted in two deaths. ESBLKp isolates were subtyped by pulsed field gel electrophoresis, and four of the five isolates typed were identical. Contro...

Napjaink egyik legégetőbb témája a környezetvédelem, amely egyre több vállalati promóciós tevékenység során megjelenik. Az internet térnyerése, digitális világ és közösségi média előtörése újabb lehetőségeket nyitott vállalatoknak zöld tevékenységek folytatásához. A tanulmány célja, hogy megvizsgálja az online térben fogyasztók hozzáállását cégek környezetvédelemmel kapcsolatos hirdetéseihez kü...

A fenntartható élelmiszer-fogyasztás jövőbeli trendjei közé tartozik többek között a rövid élelmiszer-ellátási láncok terjedése, amely párosul fogyasztói magatartás megváltozásával: például termékinformációk iránti növekvő érdeklődés, helyi termelők és helyben előállított élelmiszerek bizalom. szakirodalom egyre többet foglalkozik vagy alternatív élelmiszerekkel, amelyek vásárlói „fekete dobozn...

Agricultural support has changed substantially in both rich and poor countries recent years. In countries, there been a strong move to decoupled subsidies fall average rates of protection. developing market price remains the dominant form protection, have risen—breaking traditional pattern taxing agriculture. Emissions from agriculture land use change contributed up third total greenhouse gas e...

Aim—To assess the cell mediated immune response to BCG vaccine in preterm babies. Methods—Sixty two consecutive preterm babies born at < 35 weeks of gestation were randomly allocated into two groups. Babies in group A were vaccinated early at 34–35 weeks and group B were vaccinated late at 38–40 weeks of postconceptional age. The two groups were similar in terms of: gestational age (mean (SD) 3...

This study is relevant towards determining the ability of Turkey in mitigating environmental degradation which one major areas concern. Therefore, we aimed to examine linkage among agriculture, energy use, economic performance and FDI with sustainability. To this end, ecological footprint has been considered as a more comprehensive reliable indicator measure level degradation. relationship inve...

In peroxisomes, peroxins (PEXs) 3 and 19 are the principal protein components of the machinery required for early peroxisomal biogenesis. For further insight into the interaction of PEX3 and PEX19, we used hydrogen exchange mass spectrometry to monitor conformational changes during complex formation between PEX3 and PEX19 in vitro. Our data showed that PEX19 remained highly flexible during inte...

LdtR is a transcriptional activator involved in the regulation of a putative L,D transpeptidase in Liberibacter asiaticus, an unculturable pathogen and one of the causative agents of Huanglongbing disease. Using small molecule screens we identified benzbromarone as an inhibitor of LdtR activity, which was confirmed using in vivo and in vitro assays. Based on these previous results, the objectiv...