Fatty acid binding protein was purified from renal medulla, and its binding activity and fatty acid composition were determined in spontaneously hypertensive stroke-prone rats (SHRSP). Wistar-Kyoto rats (WKY) were used as controls. Fatty acid binding activity was higher in 5-week-old prehypertensive SHRSP than in control WKY (0.155 +/- 0.006 vs 0.030 +/- 0.001 mol palmitic acid/mol protein). Ho...

Submit Manuscript | http://medcraveonline.com Abbreviations: PUFA: Polyunsaturated Fatty Acid; PCR: Polymerase Chain Reaction; DNA: Deoxyribonucleic Acid; RNA: Ribonucleic Acid; SFA: Saturated Fatty Acid; MUFA: Monounsaturated Fatty Acid; SCD: Δ9-desaturase; FASN: Fatty Acids Synthase; STAT5: Signal Transducer and Activator of Transcription 5; CEBPA: CCAAT/Enhancer-Binding protein (alpha); CLA:...

Recently, we identified the two myeloid related protein-8 (MRP8) (S100A8) and MRP14 (S100A9) as fatty acid-binding proteins (Klempt, M., Melkonyan, H., Nacken, W., Wiesmann, D., Holtkemper, U., and Sorg, C. (1997) FEBS Lett. 408, 81-84). Here we present data that the S100A8/A9 protein complex represents the exclusive arachidonic acid-binding proteins in human neutrophils. Binding and competitio...

circulation and represent an important, readily available energy source for certain organs like liver and heart. Controversy exists over how they are translocated across the plasma membrane [ 13. Since albumin is not taken up into the cells, only the dissociated unbound fatty acids are available for uptake. For evaluation of whether this uptake occurs by simple diffusion or facilitated transpor...

1. A new, simple and high-yield procedure is described for the purification of hepatic fatty-acid-binding protein from rat liver using naphthylaminodecyl-agarose as an affinity column. 2. Cysteine-69 is shown to react slowly, but quantitatively, with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), indicating that the thiol group is free, but may be buried within the protein. 3. Fatty acids do not ...

AIM 2,3,5,4'-Tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG), a natural stilbene, shows great activities in hepatic lipid regulation, especially for hepatic triglyceride lowering. However, information about its mechanisms on biosynthesis and degradation of triglyceride is still limited. This research pays close attention to clarify the mechanism of TSG on prevention of hepatic lipid accumulation....

References 1. Matarese V, Stone RL, Waggoner DW, Bernlohr DA. Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins. Prog Lipid Res 1989;28:245–72. 2. Baier LJ, Sacchettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, et al. An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increase...

an 8-week feeding experiment was carried out on beluga (huso huso) juvenile to evaluate the effects of different levels of fish oil containing n-3 highly unsaturated fatty acids (n-3 hufas) on fish growth and fatty acid composition. the requirements of beluga juvenile for n-3 hufas were studied by feeding fish diets containing six different levels of n-3 hufas ranging from 1.56 to 17.25% (% of ...