The purpose of the present study was to evaluate the functional and biochemical effects of serum concentrations on hypothermic preservation of cardiac myocytes. Myocytes were isolated from neonatal rat ventricles and cultured for 4 days, and then incubated for 24 hrs at 4 degrees C in media containing 0, 2, 5, 10, and 20% fetal calf serum (FCS). The myocytes were then cultured for an additional...

We examined the effect of fetal calf serum (FCS) on meiotic division, subsequent fertilization, and first cleavage to the 2-cell stage of rat oocytes during in vitro maturation. FCS had no effect on the nuclear progression from dictiate to metaphase of the second maturation in vitro and, FCS had no effect on the first cleavage to the 2-cell stage of fertilized oocytes. However, FCS efficiently ...

Dendritic/tumor fusion cell (FC) vaccine is an effective approach for various types of cancer but has not yet been standardized. Antitumor activity can be modulated by different mechanisms such as dendritic cell (DC) maturation state. This study addressed optimal strategies for FC preparations to enhance Ag-specific CTL activity. We have created three types of FC preparations by alternating fus...

Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with fluorescence correlation spectroscopy (FCS). STED-FCS combines the diffraction-unlimited sp...

Fluorescence correlation spectroscopy (FCS) is a sensitive technique commonly applied for studying the dynamics of nanoscale-labeled objects in solution. Current analysis of FCS data is largely based on the assumption that the labeled objects are stochastically displaced due to Brownian motion. However, this assumption is often invalid for microscale objects, since the motion of these objects i...

Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We fo...

Previously we described that bacterial lipopolysaccharide (LPS) promoted DNA synthesis and supported the cell viability in the factor-dependent macrophage cell lines BDM-1 and BDM-1W3 in the absence of colony-stimulating factor (CSF). To further examine this phenomenon, in the present study we examined the effects of serum on CSF-dependent proliferation and LPS-induced DNA synthesis in BDM-1 an...

FCS 840 Community, Societal, and International Research 3(3,0) Covers issues and methods of community, societal, and international research and evaluation; macro-level assessment of the impact of interventions and the documentation of change; multi-method etic and emic strategies, theory-based evaluation, longitudinal designs, and collaborative, consultative models of research. Preq: PSYCH 810,...

Existing real-time ORB middleware standards such as RT-CORBA do not adequately address the challenges of 1) providing robust performance guarantees portably across different platforms, and 2) managing unpredictable workload. To overcome this limitation, we have developed software called FCS/nORB that integrates a Feedback Control real-time Scheduling (FCS) service with the nORB small-footprint ...

The UWB (Ultra Wide Band) technology can play an effective role in FCS (Fieldbus Control System). But the channel acquisition time of UWB is high, and a lot of packets are short in FCS. So the efficiency of FCS based on UWB will significantly reduce. In this paper, a CSMA/CA protocol with D-UF (Dynamic Length Mechanism for Unite Frame) is proposed. The characteristics of UWB and size of data pa...