OBJECTIVE The present study was conducted to investigate the seroprevalence of Toxoplasma gondii infection in water buffaloes. METHODS The research was conducted on 131 water buffaloes from Samsun and Afyon provinces. Serum samples of buffaloes were tested for toxoplasmosis with the Sabin Feldman Dye Test (SFDT), using live tachyzoites and methylene-blue dye. RESULTS 87.79% of buffaloes had...

The dye test for the detection of Toxoplasma-specific antibodies was first described by Sabin and Feldman 50 years ago. The test is highly specific and sensitive and considerable information is available on the development and persistence of dye test antibodies after primary Toxoplasma infection. However, the test uses live Toxoplasma gondii and is now only employed in a few laboratories. It is...

Toxoplasmosis is a zoonosis that affects both animals and humans worldwide. The aim of the present study was to compile initial epidemiological data on the prevalence of Toxoplasma gondii antibodies in Tabriz area (Northwest Iran) sheep. In a Cross-sectional study, 181 sheep blood samples from 17 different regions flocks were obtained with a sterile venoject from jugular vein. Samples were tran...

AN opportunity presented itself in September and October, 1958, to observe African patients with chorio-retinitis in Kano, Nigeria. Although it was not possible to carry out detailed studies on these patients, blood sera were taken and tested for antibodies to Toxoplasma, a protozoan parasite. The results of these tests are of interest. Wolf, Cowen, and Paige (1938) established toxoplasmosis as...

Infections of Eimeria stiedae in normal and splenectomized rabbits and of Leishmania enriettii in normal rabbits and guinea-pigs and in splenectomized rabbits did not produce dye test antibodies. Guinea-pigs injected with Atoxoplasma sp. and rabbits immunized with cultures of Crithidia fasciculata were found to be negative for dye test antibodies. Mice which had been infected with Trypanosoma c...

Using the Sabin-Feldman dye test, sera from wild and domestic animals in New Mexico, Arizona and Colorado were tested for the prevalence of antibodies to Toxoplasma gondii. The prevalence of positive titers (greater than or equal to 1:8) in animals from these areas was: New Mexico (178 of 569, 31%), Arizona (11 of 56, 20%), and Colorado (2 of 7, 29%). The overall prevalence of antibodies to Tox...

Sera collected from 63 sheep older than one year of age in two regions of Yalova were tested for anti- Toxoplasma gondii antibodies using the Sabin-Feldman Dye Test (SFDT) and Latex Agglutination Test (LAT). Of the 63 samples tested, 42 (66.66%) and 41 (65.08 %) were determined to be seropositive by SFDT and by LAT, respectively. Of the positive sheep serum samples, 23 were positive at a diluti...

A method that increases the sensitivity and specificity of the direct agglutination (AG) test for diagnosis of Toxoplasma gondii infection is described. Qualitative results in the Sabin-Feldman dye test (DT) and AG test were in excellent agreement (98%). Differences in titers between these two tests often related to the length of time the individual was infected. The AG test titer was most ofte...

The aim of this study was to determine the seroprevalence of Toxoplasma gondii in horses bred for sportive purposes in the province of Ankara. Sera collected from a total of 100 clinically healthy horses were tested for the presence of anti-Toxoplasma gondii antibodies using the Sabin-Feldman dye test and a seropositivity of 28% was found. The distribution of seropositivity among dilutions show...