BACKGROUND Routine staining procedures often pose a problem in differentiating a mitotic cell from an apoptotic cell, deteriorating the reliability of histology grading. Although various new methods have been recommended for identifying mitotic figures (MFs) in tissues, the time factor and cost makes them less feasible. Thus, an attempt was made to evaluate the efficacy of crystal violet and Fe...

An adaptation of the Feulgen procedure to visualise DNA at the ultrastructural level, using osmium ammine instead of Schiff reagent, was applied to ultrathin sections of rabbit epididymal spermatozoa, known to display increasing chromatin compaction as they progress through the epididymis. In contrast with the somatic cell nuclei of the epididymal epithelium, which display classical staining, t...

OsO(4) has many advantages over Carnoy's fixative mixture for the Feulgen nuclear staining in the protozoan Tokophrya infusionum. While Carnoy's fluid used prior to the Feulgen reaction produces shrinkage of the macronucleus and coarse clumping of its chromatin bodies, OsO(4) preserves faithfully the size and shape of the macronucleus and its chromatin material. This finding seems to be of spec...

A Computer-aided cytogenetic method for the diagnosis of breast cancer is proposed. The approach is based on statistical analysis of indexes of interphase nuclei of buccal epitheliocytes, calculated with respect to their RGB-image after Feulgen staining.

A Computer-aided cytogenetic method for the diagnosis of breast cancer is proposed. The method is based on mathematical/statistical analysis of the indexes of interphase nuclei of buccal epitheliocytes, calculated with respect to their RGBimage after Feulgen staining.

in this study the role of cell death in cartilage canal morphogenesis with morphometrical and histological methods was evaluated. the proximal tibial epiphysis of the chick embryos after fixation and decalcification at the 13 th and 18th days of incubation, with thick and semithin sections and h&e; ,tricromason and feulgen staining were studied. the rsulths were showed that cartilage canals wer...

The composition, with respect to nucleic acids, of the L.E. bodies resulting from the action of the plasma of patients with systemic lupus erythematosus on substrate leukocyte nuclei in different kinds of preparations was compared microspectrophotometrically with that of control lymphocyte nuclei. Binding of the basic dye methyl green to DNA was uniformly found to be depressed in L.E. bodies as...

The micronucleus (MN) assay in human exfoliated cells has been widely used to detect the genotoxic effects of environmental mutagens, infectious agents and hereditary diseases. Substantial variability characterizes the MN frequencies reported by different research groups. One reason for this may be the restricted resolution power of the Feulgen-Fast-Green staining that is routinely used. Here w...