Results obtained with a potentiometric analyzer, NOVA 1, specific for sodium and potassium, were compared with those by flame photometry. Both instruments showed linearity within a physiological range of sodium and potassium concentrations and had similar precisions. Volume displacements from addition of albumin or Intralipid to aqueous samples yielded the predicted lower flame-photometric resu...

The concentrations of sodium, potassium, and chloride in various control sera were determined by reference methods. The reference method values were compared with the corresponding method-dependent assigned values. Sodium: Measurements by flame photometry and ion selective electrodes differed on the whole by less than 1% from the reference method value; determinations by photometry differed, ho...

Serum sodium and potassium analyses by continuous-flow flame photometry is a well-accepted procedure (1, 2). Recently Habig and Williamson (3) have used the widely available Instrumentation Laboratory Model 143 (IL-143) flame photometer for continuousflow analyses. Pennacchia et al. (4) have operated this flame photometer at rates as high as 120 samples per hour. In both systems the results app...

At the present time, the applicability of the flame photometer to the clinical laboratory for determination of the cations sodium, potassium, and calcium in biological fluids has been demonstrated by numerous investigators (l-7). The determination of magnesium in blood serum and plasma by flame photometry has also been described (2, 8). However, analysis of physiologically significant anions in...

The need for a rapid, accurate method to determine quantitatively Na and K present together in biological fluids has been met by the procedures to be described. The necessity of separating these elements prior to their determination by the various chemical methods usually employed has led to procedures which are often prohibitively tedious and time-consuming. The recent development of flame pho...

With electrolyte reference fluid (ERF)00, results from Kodak Ektachem slides for the direct potentiometric assay of sodium in plasma were significantly correlated with results from flame photometry, but also appeared to be systematically higher, especially in hypernatremic patients. Indirect potentiometry with the Technicon RA-1000 yielded intermediate values. In 23 hypernatremic patients with ...

The exchange (release resp. uptake) of K+, Rb+, and Na+ ions in the isolated retina of the crayfish Astacus leptodactylus with the surrounding medium was measured using flame photometry and radioisotopic techniques. 1. The net release of K+ into a Na+ and K+ free, choline containing medium, as measured by means of flame photometry, is increased by illumination. 2. The release of 86Rb+, used as ...

Flame photometry has many applications, and in the clinical field its use in the estimation of serum sodium and potassium is now well established. Difficulties have been encountered in the estimation of serum calcium by flame photometry, since the emission spectrum of calcium is relatively weak and is subject to interference by the presence of other ions, e.g., sodium. Although many methods hav...

Shyr and Young recently published a better concerning falsely bow values for sodium and potassium obtained when using flame photometry including sample dilution (Clin. Chem. 26: 1517, 1980). I have found that falsely low values occur when using the IL-144 autodilutor and aqueous calibrating solutions, mostly because of differences between serum and calibrator viscosity, i.e., the calibrators mo...