background: acanthamoeba keratitis (ak) is potentially a sight-threatening infection and its treatment is challenging. this is mainly due to presence of resistant cyst form. indeed, cysts are highly resistant to current available drugs. chemical drugs are toxic to human keratocytes. it should also be mentioned that most available anti- acanthamoeba drugs are poorly cysticidal, in iran and world...

objective(s) in the present study, the differences in the relaxant effects of aqueous, ethyl acetate and n-butanol fractions of rosa damascena on tracheal smooth muscle of guinea pigs were examined. materials and methods the relaxant effects of three cumulative concentrations of each fraction (0.1, 0.2 and 0.4 g%) in comparison with saline and three cumulative concentrations of theophylline (0....

the chemical speciation of heavy metals (arsenic, chromium, copper, lead, zinc and tin) in the sediments offormer tin mining catchment bestari jaya, peninsular malaysia was determined by using the latest version of the community bureau of reference, usually called the bcr 3-step sequential extraction procedure. furthermore, a fourth step was introduced for digestion and analysis of the residue....

The lipid class compositions of subcellular fractions of SV40-transformed mouse fibroblasts derived from the logarithmic and stationary growth phase were compared. Cell populations of the stationary growth phase showed a relative decrease of the protein content and an increase of triglycerides and alkoxydiglycerides which could be located in the non-sedimenting fraction and in the nuclei and mi...

In etiolated pea (Pisum sativum L. cv. Alaska) shoots about 3% of the total extractable phytochrome was found in the mitochondrial fraction and about 4.5% in the microsomal fraction, while over 70% was soluble in the 105,000g supernatant. The value of Delta(DeltaA) per milligram of protein was significantly higher in the 105,000g supernatant than in these particulate fractions. The percentage c...

The formaldehyde-killed, whole-spherule vaccine, which is protective against lethal challenge of laboratory animals with Coccidioides immitis, was fractionated. It yielded a soluble, multicomponent, subcellular fraction termed the 27K vaccine. This vaccine, when it was accompanied by adjuvant, protected mice against lethal intranasal and intravenous challenge with C. immitis.

Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of investigators. Here we present and discuss the work of several groups that have obtained highly purified subcellular fractions from trypanosomatids, Apicomplexa and trichomonads, and whose work have added substantially to our knowledge o...

objective: the use of drugs with herbal origin is increasing for treatment of gastrointestinal (gi) disorders. rosa damascena (r. damascena) is a well-known plant suggested to have beneficial effect on gi system. in this study, the effect of aqueous fraction of r. damascena on the contractionsofisolated guinea pig ileum was investigated. materials and methods: aqueous fraction of plant was obta...

background : within the framework to control mosquitoes, ovicidal, larvicidal and pupicidal activity of annona senegalensis leaf extract and its 4 fractions against anopheles gambiae and culex quinquefasciatus were evaluated in the laboratory conditions. methods : ovicidal test was performed by submitting at least 100 eggs of mosquitoes to 125, 250, 500, 1000 and 2000 ppm concentrations, while ...

the present experiment was carried out to determine the digestion kinetics of carbohydrate fractions of citrus by-products. grapefruit pulp (gp), lemon pulp (le), lime pulp (li) and orange pulp (op) were the test feed. digestion kinetic of whole citrus by-products and neutral detergent fiber (ndf) fraction and acid detergent fiber (adf) fractions of citrus by-products were measured using the in...