The incorporation of [3H]fucose into glycoproteins of Sarcoma 180 cells in vitro was decreased within 2 hr of exposure to 6-thioguanine (6-TG); 50% inhibition was pro duced by 8 /nM 6-TG. Under identical conditions, no de crease in [3H]glucosamine incorporation into acid-insoluble material was detected. Similarly, [3H]fucose incorporation, but not [14C]glucosamine incorporation into glycoprotei...

India contributes a major pool of head and neck cancer (HNC) patients in global cancer burden. Measurement of L-fucose glycoprotein has been used as a cancer biomarker. In this study, preoperative levels of serum L-fucose glycoprotein were compared with that of postoperative levels measured at 3rd week in 50 HNC patients without distant metastasis. The mean value of serum glycoprotein L fucose ...

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vi...

PURPOSE Recent studies have revealed that fucose removal from the oligosaccharides of human IgG1 antibodies results in a significant enhancement of antibody-dependent cellular cytotoxicity (ADCC) via improved IgG1 binding to FcgammaRIIIa. In this report, we investigated the relationship between enhanced ADCC and antigen density on target cells using IgG1 antibodies with reduced fucose. EXPERI...

GDP-fucose transporter plays a crucial role in fucosylation of glycoproteins by providing activated fucose donor, GDP-fucose, for fucosyltransferases in the lumen of the Golgi apparatus. Fucose-containing glycans are involved in many biological processes, which are essential for growth and development. Mutations in the GDP-fucose transporter gene cause leukocyte adhesion deficiency syndrome II,...

Studies concerning the effects of naturally occurring simple sugars on the morphology and metabolism of various mam malian cell lines further illustrate the selective effects of differ ent individual sugars on specific cell lines. Attempts to char acterize the selective effects of L-fucose on 3T3 fibroblasts showed that the marked changes in morphology and inhibition of incorporation of radioac...

D-Arabinose is degraded by Escherichia coli B via some of the L-fucose pathway enzymes and a D-ribulokinase which is distinct from the L-fuculokinase of the L-fucose pathway. We found that L-fucose and D-arabinose acted as the apparent inducers of the enzymes needed for their degradation. These enzymes, including D-ribulokinase, appeared to be coordinately regulated, and mutants which constitut...

The protozoan parasite Trypanosoma brucei causes human African sleeping sickness in sub-Saharan Africa. The parasite makes several essential glycoproteins, which has led to the investigation of the sugar nucleotides and glycosyltransferases required to synthesize these structures. Fucose is a common sugar in glycoconjugates from many organisms; however, the sugar nucleotide donor GDP-fucose was...

Pea microsomal membranes catalyze the transfer of [(14)C]fucose (Fuc) from GDP-[U-(14)C]fucose, with or without added unlabeled UDP-glucose (Glc), UDP-xylose (Xyl) or UDP-galactose (Gal), to an insoluble product with properties characteristic of xyloglucan. After digestion of the ethanol-insoluble pellet with Streptomyces griseus endocellulase, [(14)C] fucose residues occur exclusively in a fra...

enzyme was purified to homogeneity as indicated by a single band in disc-gel electrophoretic columns and single peaks with column chromatography and ultracentrifugation from the wild-type PRL-R3 strain, induced with L-fucose and two constitutive mutants, 502 and 510. The ratios of the activities of this isomerase on Darabinose and L-fucose remained constant throughout all purifications. The app...