We have evaluated the use of allele-specific PCR (AS PCR) on DNA pools as a tool for screening inherited genetic variants that may be associated with risk of adult acute myeloid leukemia (AML). Two DNA pools were constructed, one of 444 AML cases, and another of 823 matched controls. The pools were validated using individual genotyping data for GSTP1 and LTalpha variants. Allele frequencies for...

Warfarin is widely prescribed for patients with the risk of thromboembolism around the world. The inter-individual and inter-racial differences in appropriate dosage depend highly on age, body weight, and genetic factors. A lot of studies including genome-wide association studies revealed that vitamin K epoxide reductase complex, subunit 1 (VKORC1) G-1639A and Cytochrome P450 (CYP) 2C9 A1075C a...

Rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. Rotavirus G and P genotyping is essential for epidemiological surveillance and for better formulation of candidate rotavirus vaccines. Out of 862 diarrheal stool samples collected from hospitalized children aged < 2 years during February 2005 - March 2007, 318 (36.9%) were positive for rotavirus by ...

Resistin (RETN), recently found to be relevant to inflammation and inflammatory disorders. We, therefore, aimed to investigate the potential role of RETN gene polymorphism in pathogenesis of acne vulgaris with familial history. We investigated the RETN-420C/G polymorphism in 180 patients with acne vulgaris and 180 healthy individuals in a case-control association analysis. In this study, we als...

Hepatitis B virus (HBV) genotyping has become important in epidemiological and clinical diagnoses, given the relationship between the viral genotype and the progression of disease or the appearance of antiviral resistance. Since genotyping by sequence and phylogenetic analyses is not convenient in the clinical setting, we evaluated InnoLipa HBV genotyping (Innogenetics, Belgium) and an HBV DNA-...

objective(s):organic anion transporter polypeptide 1b1 (oatp1b1) encoded by solute carrier organic transporter 1b1 (slco1b1) gene; a transporter involved in the uptake of drugs and endogenous compounds is present in hepatocyte sinusoidal membrane. aim of this study was to investigate the frequencies of functionally significant snps (388a>g and 521t>c) and their haplotypes in 6 ethnic groups of ...

background: giardia duodenalis is one of the most common human intestinal pro-tozoan parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. the present study aimed to identify the prevalence of g. duo-denalis isolates and determine the most common of its assemblages in the patients referring to health centers and hospitals in fars province, iran that will ...

with the LightCycler. Clin Chem 1999;45:694–6. 15. Hiratsuka M, Agatsuma Y, Mizugaki M. Rapid detection of CYP2C9*3 alleles by real-time fluorescence PCR based on SYBR green. Mol Genet Metab 1999;68:357–62. 16. Aslanidis C, Schmitz G. High-speed apolipoprotein E genotyping and apolipoprotein B3500 mutation detection using real-time fluorescence PCR and melting curves. Clin Chem 1999;45:1094–7. ...

Genotyping of individuals 7 commercial breeds domestic pigs was carried out: Duroc, Landrace, Belarusian Large White, Black-and-White, meat, Pietrain and Yorkshire for the KDM3A DBX2 genes. The high breed-specific potential polymorphic loci g.58335217A&gt;G (KDM3A gene) g.75953650G&gt;T (DBX2 differentiation Duroc species Sus scrofa domesticus confirmed. A test model by bioinformatic assessment...