?-Galactosidases of Mucoromycota are rarely studied, although this group filamentous fungi is an excellent source many industrial enzymes. In study, 99 isolates from the genera Lichtheimia, Mortierella, Mucor, Rhizomucor, Rhizopus and Umbelopsis, were screened for their ?-galactosidase activity using a chromogenic agar approach. Ten best producers selected, was further investigated in submerged...

Beta-galactosidase (ß-D-galactoside galactohydrolase, EC 3.2.1.23), has tremendous potential in research and application in various fields like food, bioremediation, biosensor, diagnosis and treatment of disorders. The sources of the betagalactosidase are microorganisms, plants and animals. Microbial beta-galactosidase is of much interest because of their thermostability, thermoacidophilic and ...

In this work we report a detailed analysis of the topology and phylogenetics of family 2 glycoside hydrolases (GH2). We distinguish five topologies or domain architectures based on the presence and distribution of protein domains defined in Pfam and Interpro databases. All of them share a central TIM barrel (catalytic module) with two β-sandwich domains (non-catalytic) at the N-terminal end, bu...

Our studies with purified human liver acid 6-D-galactosidases (EC 3.2.1.23) indicate that 4-methylumbelliferyl fi-D-galactosidase and GM1-ganglioside /-D-galactosidase activities are identical with lactosylceramidase II activity. Evidence for this includes co-purification of all enzyme activities by affinity chromatography to yield a single band on polyacrylamide-gel electrophoresis and coincid...

A new type of galactose mimetics has been synthesized following a straightforward synthetic approach based on cyclohexene olefin aziridination reactions directed by hydroxyl substituents. These enantiomerically pure galacto-configured N-aminoaziridines are potent irreversible inhibitors of Aspergillus oryzae and Escherichia coliβ-galactosidases.

A gene encoding a third alpha-galactosidase (AglB) from Aspergillus niger has been cloned and sequenced. The gene consists of an open reading frame of 1,750 bp containing six introns. The gene encodes a protein of 443 amino acids which contains a eukaryotic signal sequence of 16 amino acids and seven putative N-glycosylation sites. The mature protein has a calculated molecular mass of 48,835 Da...

the rose sawfly, arge rosae linnaeus, is one of the most destructive pests of rose bushes in the north of iran. nowadays, many attempts have been made to reduce pesticide application by looking for new methods of pest control. a non chemical method for controlling insect pests including a. rosae can be achieved by using genetically engineered plants expressing carbohydrase inhibitors. therefore...