The fractional rates of catabolism of isotopically labeled mouse, human, bovine, and guinea pig gamma-globulins and human serum albumin were determined in mice and in guinea pigs whose serum gamma-globulin and serum albumin levels were elevated by immunization or by injections of exogenous serum proteins. These serum proteins were also followed in mice with different serum gamma-globulin levels...

Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated gamma-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of gamma-globulin. The bulk of this gamma-globulin, identified as gamma(G)-globulin, was homogeneous by zone electrophoresis and of specificity dire...

Normal 7S human gamma-globulin was found to contain two fundamental antigenic groups of molecules. The group 1 molecules of normal gammaglobulin correspond antigenically to group 1 multiple myeloma proteins and Bence Jones proteins; and group 2 molecules of normal gamma-globulin correspond antigenically to group 2 multiple myeloma proteins and Bence-Jones proteins. Among pooled human Fr II and ...

The cellular sites of formation of gammaglobulin in lymphatic tissues of man and in a representative human lymphoid infiltrate have been studied by fluorescent antibody technique. The findings indicate that gamma-globulin is formed in the germinal centers of lymphatic nodules and in the cytoplasm of mature and immature plasma cells of two types-those with and those without Russell bodies. The g...

Gamma globulin formation in vitro by various tissues was studied using the incorporation of C(14)-L-lysine into a protein precipitable by a specific anti rabbit gamma globulin serum prepared in a sheep. It was demonstrated that the rate of gamma globulin formation in similar numbers of spleen cells is much higher if taken from an immunized rabbit at the height of antibody formation than that in...

Studies on antibody formation "in vitro" have supplied information concerning the site of antibody production (1-5), the rates of antibody synthesis per unit weight of tissue (6-7) and the percentage of active cells in lymphoid tissue during the primary and secondary antibody response (8-9). In vitro experiments have also been used in attempts to elucidate the mechanism of induction of antibody...

Gamma globulin and antibody obtained from inbred C(3)H mice are split by papain and cysteine into fragments roughly one-third the size of the original Molecule (S(20,w) = 3.5S). The papain digests were characterized by starch gel electrophoresis and immunological methods. The highly heterogeneous fragments could be divided into two groups with distinct antigenic determinants (S and F), which we...

The effect of highly purified rheumatoid factor on the precipitin reactions of various antigen-antibody systems was determined. The amount of nitrogen precipitated was increased over a broad range when the factor was added to ovalbumin, human albumin, or human gamma globulin, and the corresponding rabbit antibodies. In the zone of antigen excess, soluble antigen-antibody complexes were precipit...

Many sera from normal individuals as well as patients with various disease states contain agglutinating antibodies which show specificity for antigenic determinants of gamma-globulin revealed by pepsin digestion at pH 4.1. Sera containing such agglutinating activity as well as sera negative for these agglutinators contain low molecular weight (3S-5S) components of slow gamma-mobility which inhi...