Pepstatin, a strongly bound inhibitor of acid proteases, does not instantaneously bind to pepsin. The dissociation constant of pepsin-pepstatin complex is 9.7×10-11 M using Phe・Gly・His・Phe(NO2)・Phe・Ala・PheOMe as substrate. Difference ultraviolet absorption spectra show conformational change of pepsin in interaction with pepstatin. The bindillg of pepsin with pepstatin is stoicheometric and the ...

objective(s) the liver has major role in the organism homeostasis, interactions with other systems, synthesis and metabolism of bile production, drug detoxification and hormone inactivation. cholestasis can be defined as an impairment of the bile flow which can lead to hepatocytes necrosis and finally cirrhosis. some studies reported a gastric acid secretion reduction in cirrhotic subjects, whi...

Objective(s) Gastric ulceration is induced by various forms of stress like surgery, ischemia and trauma. The female sex has more resistance to stress and the gastrointestinal lesions happen fewer than male sex. The purpose of this study was to evaluate the role of estradiol and progesterone on the gastric acid and pepsin levels following traumatic brain injury (TBI) induction. Materials and M...

Crystalline proteins, such as edestin or melon globulin, remove pepsin from solution. The pepsin protein is taken up as such and the quantity of protein taken up by the foreign protein is just equivalent to the peptic activity found in the complex. The formation of the complex depends on the pH and is at a maximum at pH 4.0. An insoluble complex is formed and precipitates when pepsin and edesti...

1. Fresh and inactivated animal serum under proper conditions will bind pepsin quantitatively in weak acid solution and will prevent it from digesting proteid even after the addition of free hydrochloric acid in excess. 2. This binding and inactivation of pepsin cannot be considered as due to a specific anti-pepsin. 3. The phenomenon has been named pepsin deviation in analogy with the deviation...

objective(s) gastric ulceration is induced by various forms of stress like surgery, ischemia and trauma. the female sex has more resistance to stress and the gastrointestinal lesions happen fewer than male sex. the purpose of this study was to evaluate the role of estradiol and progesterone on the gastric acid and pepsin levels following traumatic brain injury (tbi) induction. materials and met...

BACKGROUND Extra-esophageal carcinogenesis has been widely discussed in relation to the chronic effects of laryngopharyngeal reflux and most prominently with pepsin historically central to this discussion. With refluxate known to include gastric (pepsin) and duodenal (bile) fluids, we recently demonstrated the mechanistic role of NF-κB in mediating the preneoplastic effects of acidic-bile. Howe...

A comparison has been made of the relative velocity of hydrolysis of the various peptid linkings of the gelatin molecule when hydrolyzed by acid, alkali, pepsin or trypsin. It has been found that: 1. Those linkages which are most rapidly split by pepsin or trypsin are among the more resistant to acid hydrolysis. 2. Those linkages which are hydrolyzed by pepsin are also hydrolyzed by trypsin. 3....