A new macromolecular antibiotic C-1027 was obtained from the broth filtrate of Streptomyces globisporus C-1027 by precipitation with ammonium sulfate, DEAE-cellulose column chromatography and gel filtration chromatography on a Sephadex G-75 column. This antibiotic, prepared as a white powder, is an acidic polypeptide having an isoelectric point of pH 3.5-3.7 and a molecular weight of 15,000 as ...

We describe methods for studying the binding of Al by protein in serum: ultrafiltration, gel filtration, and immuno-affinity chromatography. For ultrafiltration we used an Amicon YM10 cellophane membrane with a nominal cutoff of 10 000 Da to separate ultrafiltrable and non-ultrafiltrable Al. For gel filtration we used Sephacryl S-300, and for immuno-affinity chromatography we used anti-transfer...

A Glycoprotein, particularly high in tumors, has been extracted from Morris 5123C rat hepatomas and purified. The compound constitutes a major binding component for 67Ga in this hepatoma. It has a molecular weight of approximately 45,000. Its molecular weight was determined by sodium dodecyl sulfate:polyacrylamide gel electrophoresis and by Sephadex G-200 superfine gel filtration. The steps inv...

The lectin of Limulus polyphemus haemolymph agglutinates erythrocytes [l-4] and other cells [ 51. Neuraminic acid has been shown to be the specific ligand of this lectin. The lectin has been isolated by ultracentrifugation and preparative starch gel electrophoresis [6], by DEAE-Sephadex and gel filtration chromatography [7] and by ultracentrifugation, affinity and gel filtration chromatography ...

Background: The chaperone activity of Mycobacterium tuberculosis Acr is an important function that helps to prevent misfolding of protein substrates inside the host, especially in conditions of hypoxia. Objectives: The aim of this study was to establish the correlation of structure and function of recombinant Acr proteins both before and after ge...

a blocker of postsynaptic acetylcholine receptors was isolated from venom of the eastern green mamba dendroaspis angusticeps, by gel filtration, ion-exchange and reverse phase high performance liquid chromatography. the isolated component blocks neuromuscular transmission and responses to exogenously applied acetylcholine in isolated chick biventer cervices preparations. these results suggest t...

Methods The three storage proteins were all purified from unpolished sesame seeds extracted at pH 7,5. The 11S globulin and the 7S vicilin were enriched by precipitation in water. Pure 11S globulin was obtained by a further precipitation with 35% ammonium sulfate followed by anion exchange chromatography. Pure 7S vicilin was obtained by adding gel filtration and anion exchange chromatography. T...