Apparatus necessary for a quantitative gel diffusion and precipitation assay of duck hepatitis virus was designed and constructed.

This paper describes an allotypic system in cattle called Ec (Erythrocyte cattle). The antigenic determinant is derived from red cells and is detected in lysates by gel precipitation with alloimmune serum. The Ecl specificity is controlled by an autosomal dominant gene and occurs with variable frequencies in different cattle breeds. The Ec system is a very useful genetic marker because the homo...

Methods The three storage proteins were all purified from unpolished sesame seeds extracted at pH 7,5. The 11S globulin and the 7S vicilin were enriched by precipitation in water. Pure 11S globulin was obtained by a further precipitation with 35% ammonium sulfate followed by anion exchange chromatography. Pure 7S vicilin was obtained by adding gel filtration and anion exchange chromatography. T...

A bacterial factor preventing precipitation of immune complexes was purified from a culture of Streptococcus faecalis (AK strain) by gel filtration on Sephadex G-100 and Bio-Gel A-150m columns. The factor was identified as DNA on the basis of its ultraviolet absorption maximum at 260 nm, hydrolysis by deoxyribonuclease, Mr exceeding 1.5 X 10(8), and positive reaction with diphenylamine. DNA int...

We describe a fast and easy method for routine quantitation of the abnormal lipoprotein, lipoprotein-X. The procedure is based on densitometry of precipitation areas obtained for it after serum electrophoresis in agar gel followed by precipitation with polyanions. The coefficient of variation was less than 3% in one series. Results were linearly related to concentration in the range 0.063 to 6....

To the Editor: Vieira et al. (1) used the Wallac Delfia immunofluorometric assay to demonstrate that macroprolactin is a common cause of apparent hyperprolactinemia, and this confirms our experience (2) and that of others (3). However, their data validating the polyethylene glycol (PEG) precipitation as a screening method for detecting macroprolactinemia are substantially different than ours (2...

In this paper, polyacrylamide/chromium acetate gel polymer system was prepared as water shut-off system to control water production in one of the Iranian oil reservoirs condition. Effect of various parameters such as temperature (90 °C) and water formation from view point of salinity and divalent cations, was evaluated on the gelation kinetic and gel strength using bottle testing method. Th...

in this paper, polyacrylamide/chromium acetate gel polymer system was prepared as water shut-off system to control water production in one of the iranian oil reservoirs condition. effect of various parameters such as temperature (90 °c) and water formation from view point of salinity and divalent cations, was evaluated on the gelation kinetic and gel strength using bottle testing method. the re...