نتایج جستجو برای: genomic dna
تعداد نتایج: 582362 فیلتر نتایج به سال:
This study was conducted to determine the location of DNA segment with homology to the rat conserved genomic DNA in human chromosomes. The labeled rat genomic DNA was hybridized with normal human (male) metaphases. The study of 74 metaphases after fluorescence in situ hybridization showed 371 twin-spot signals on human chromosomes. Statistical analysis indicated that the specific accumulation o...
Assisted reproductive techniques (ART) are considered as the first line of treatment in overcoming infertility. The scope of success of these methods depends on several factors including integrity of sperm and oocyte. Obviously, paternal genomic content has an important effect on fertilization potential in infertile couples. Thus, sperm DNA damage is a genomic disorder that exists in different ...
Background: Anti-dsDNA antibodies frequently found in the sera Systemic Lupus Erythematosus patients, particularly in active disease stage. Nowadays exploit different eukaryotic and prokaryotic dsDNA as antigen source and different reagents as binder. The aim of this study to compared two dsDNA different sources and tow different kinds of reagents for binder in ELISA test. Methods: In this stu...
background: deletions of the daz (deleted in azoospermia) genes within the human y chromosome's azfc region are the most common cause of spermatogenesis failure. these deletions are usually assessed by analyses of genomic dna extracted from peripheral leukocytes. daz genes are expressed in male germ cells. in this prospective study, we investigated daz expression and deletion in 102 consecutiv...
background: isolation of high quality genomic dna is one of the most important steps in molecular biology studies related to food borne parasites. usually, various protocols are used for different tissues but so far, there is no common, simple and cost benefit procedure for genomic dna extraction of linguatula serrata ( l. serrata .) larvae as a pentastomida endangering food safety. one of the ...
background identification of novel pathogenic bacteria always has a crucial role in clinical microbiology to defeat uncontrolled diseases. objectives sample preparation platform has been developed for rapid and simple detection of infectious organisms from the point of care diagnostics and molecular manipulation by using three novel genomic dna isolation protocols. materials and methods most of...
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least time and cost. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is one of the novel methods o...
this study was conducted to determine the location of dna segment with homology to the rat conserved genomic dna in human chromosomes. the labeled rat genomic dna was hybridized with normal human (male) metaphases. the study of 74 metaphases after fluorescence in situ hybridization showed 371 twin-spot signals on human chromosomes. statistical analysis indicated that the specific accumulation o...
An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism...
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