In this study we report a physical analysis of the membrane mechanics affecting the size of the highly curved region of a lipid nanotube (LNT) that is either connected between a lipid bilayer vesicle and the tip of a glass microinjection pipette (tube-only) or between a lipid bilayer vesicle and a vesicle that is attached to the tip of a glass microinjection pipette (two-vesicle). For the tube-...

It was possible to perfuse the interior of the giant axon of Loligo pealii or forbesi, [C. S. Spyropoulos, I. Tasaki and T. Teorell, National Institutes of Health Annual Report, NINDB, 1959; Baker, P. F., A. L. Hodgkin and T. D. Shaw, Preprint of Proc. Physiol. SOC. (1961); also cited in preprint, Baker, P. F. and T. D. Shaw (1961), Plymouth Marine Biological Laboratory report for 1960-1961 (in...

OBJECTIVE Leukocyte retention in lung capillaries is observed in normal physiology and following a bacterial infection. It has been hypothesized that cells either become mechanically trapped or adhere to capillary endothelial cells via adhesion molecules. We propose that retention involves both mechanical and adhesive forces and that the biochemical adhesive force is modulated by mechanical for...

Key Idea As an indispensable tool for biological investigations into cells, the micropipette allows sampling and injecting fluid across the cell membrane. This operation, however, requires a high dexterity of the operator in order not to damage the membrane and since it is a serial process, it is highly time-consuming if the number of samples grows. To increase the productivity of the sampling ...

Enhancement of XRF intensity was investigated by using an Au coated glass-capillary. Au was electroplated on an inner wall of the glass-capillary with a thickness of 100 nm. The Au coated glass-capillary was attached to a micro focus x-ray tube. The XRF intensity from the pure metal samples was measured by a silicon drift x-ray detector. It was confirmed that the intensity of XRF was enhanced b...

As a continuation of our work on boronic acid lectin affinity chromatography (BLAC), in this paper we introduce an automated affinity micropartitioning approach using combined boronic acid and concanavalin A (BLAC/Con A) resin-filled micropipette tips to isolate and enrich human serum glycoproteins. The N-linked oligosaccharides of the partitioned glycoproteins were removed by PNGase F enzyme d...

PURPOSE To quantify the distribution and morphometric characteristics of capillary networks in the human perifovea. To determine correlations between the location of neuronal subcellular compartments and the morphometric features of regional capillary networks in the layered retina. METHODS The perifoveal region, located 2 mm nasal to the fovea, was studied in 17 human donor eyes. Novel micro...

This paper proposes a method for fabricating suspended micro/nanoscale polymer fibers continuously, in which polymeric micro/nanofibers are formed by drawing and solidification of a viscous liquid polymer solution which is pumped through a glass micropipette. By controlling the drawing parameters, this method is demonstrated to form networks of suspended fibers having amorphous internal structu...