1. The removal of the beta(2)A-globulins from three sera from treated ragweed-sensitive individuals by immune absorption was associated with the loss of all detectable skin-sensitizing antibody activity as demonstrated by Prausnitz-Küstner testing. 2. Gel filtration studies, with sephadex G-200, indicated that the fractions containing only macroglobulins were devoid of all detectable skin-sensi...

By means of immunodiffusion and immunoelectrophoresis study has been made of antigenic relationships of Bence Jones proteins, and the three classes of normal and pathological immunoglobulins, 7S gamma, beta(2A), and beta(2M). All thirty-nine Bence Jones proteins studied could be classified into either one of two distinct antigenic types, A or B. Both types are related to the immunoelectrophoret...

The aim of the present study was to measure the serum total proteins and the serum protein fractions (albumin, α1-, α2-, βand γ-globulins) of ten newborn lambs (Comisana breed) during the first 30 days of life in order to obtain useful information for neonatal care. From each animal, blood samples were collected via jugular venepuncture at the same hour (9.00) every 3 days for 30 days starting ...

Present investigation reports the protein quality parameters in mungbean x ricebean (MR) and urdbean (MU) interspecific lines along with checks. Interspecific contained 138.4-230.3 mg/g total soluble proteins, 0.84-2.63 free amino acids, 3.21-7.10 methionine, 0.08-0.15 cysteine 0.67-1.32 tryptophan. Protein fractionation studies revealed 32.6-48.3 (albumins), 153.3- 218.8 (globulins), 2.81-8.30...

I T HAS BEEN KNOWN for some time that some acidified-polar organic solvents, e.g., 2% (w/v) trichloracetic acid (TCA) in ethanol, when added to serum will retain albumin in solution but precipitate all the globulins (1, 2). Several investigators have shown that albumin separated from serum in this manner is relatively free of globulins and is similar in its physical and immunologic properties t...

The role of kinins in the acute antihypertensive effect of a converting enzyme inhibitor (CEI) was studied in sodium-depleted normotensive and in two-kidney, one clip chronically hypertensive rats (2K-1C). The 2K-1C were on a normal sodium diet. The acute vasodepressor effect of the CEI was determined in these two groups either after administration of normal rabbit globulins or antikinin globul...

BACKGROUND Major portions of homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly), and glutathione in serum are covalently bound to proteins via disulfides. Albumin has been considered the dominant binding protein. METHODS Pooled serum and plasma from healthy adults were fractionated into albumin and globulins by affinity columns. Content of Hcy, Cys, CysGly, and glutathione was dete...

Certain of the physiochemical and immunochemical properties of the y-globulins of normal human urine have been reported by Webb, Rose, and Sehon (2) and by Franklin (3). These urinary proteins were found to be electrophoretically and antigenically closely related to serum y-globulins, but smaller in size; their molecular weights ranged from 10,600 (2) to 38,000 (3). Sedimentation coefficients S...