Abstract A glucose amperometric biosensor has been prepared by a multilayer deposition on glassy carbon electrode of Co3Mn-CO3 layered double hydroxide, oxidase (GOx) and biopolymer (carrageenan), respectively. The original transduction step consists in the electrocatalytic oxidation H2O2, enzymatically formed, inner-layer at 0.65 V/Ag-AgCl. sensitivity detection limit, obtained with this optim...

The surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. In this study, conformational changes of glucose oxidase (GOx) in the mercaptopurine: GNPs and 11-mercaptoundecanoic acid: GNPs as a hydrophobic and a hydrophilic GNPs surface was investigated by various spectroscopic techniques, including: UV-Vis absorption, f...

Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated impact temperature pulses on GOx that was drop-coated flattened Pt microwires. The wires were heated by alternating current. sensitivity towards and stability amperometry. An up to 22-fold increase observed. Spatially resolve...

In this work, we describe the electrochemical activation of glucose oxidase (GOx) via covalent attachment of a novel redox mediator, Os(bpy)2(API)Cl (bpy = 2,2'-bipyridine, API = 3-aminopropylimidazole), to the peptide backbone of GOx targeting at aspartate and glutamate residues. Cyclic voltammetry showed a pair of well-defined voltammetric peaks centered at 0.11 V for the activated enzyme. Os...

In Hydrogele eingebrachte chemische Reaktionsnetzwerke können responsive Materialien zu komplexen, selbstregulierenden transformieren, die eine Rückkopplung auf externe Stimuli generieren können. Diese Arbeit präsentiert aus dem Wirt-Gast-Paar β-Cyclodextrin und Ferrocen als supramolekulare Vernetzer. Ein redoxresponsives Verhalten des Systems wird induziert durch Treibstoff-Enzym-Paare Meerret...

Induction of Entner-Doudoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but n...

In this study, a simple and amplified colorimetric assay is developed for the detection of the enzymatic activity of glucose oxidase (GOx) based on in situ formation of a photoswitchable oxidase mimetic of PO₄(3-)-capped CdS quantum dots (QDs). GOx catalyzes the oxidation of 1-thio-β-d-glucose to give 1-thio-β-d-gluconic acid which spontaneously hydrolyzes to β-d-gluconic acid and H₂S; the gene...

Glucose oxidase (GOx) is an enzyme produced by Aspergillus, Penicillium and other fungi species. It catalyzes the oxidation of β-d-glucose (by the molecular oxygen or other molecules, like quinones, in a higher oxidation state) to form d-glucono-1,5-lactone, which hydrolyses spontaneously to produce gluconic acid. A coproduct of this enzymatic reaction is hydrogen peroxide (H₂O₂). GOx has found...

A simple colorimetric assay for blood glucose has been developed based on enzymatic engineering of gold nanorods by glucose oxidase (GOx)-mediated oxidative etching of gold nanorods.

Glucose sensing electrodes have been realized by immobilizing glucose oxidase (GOx) on unmodified edge plane of highly oriented pyrolytic graphite (epHOPG) and the native oxide of heavily doped silicon (SiO2/Si). Both kinds of electrode show direct interfacial electron transfer due to the redox process of the immobilized GOx. The measured formal potential of the redox process agrees with that o...