Glucosinolates are a major class of sulphur-containing secondary metabolites involved in plant defence against pathogens. Recently many regulatory links between glucosinolate biosynthesis and sulphate assimilation were established. Since sulphate assimilation undergoes diurnal rhythm and is light regulated, this study analysed whether the same is true for glucosinolate biosynthesis. The levels ...

The data presented in this article are related to the research article, "HPLC-based enzyme kinetics assay for glucosinolate hydrolysis facilitate analysis of systems with both multiple reaction products and thermal enzyme denaturation" (C.K. Klingaman, M.J. Wagner, J.R. Brown, J.B. Klecker, E.H. Pauley, C.J. Noldner, J.R. Mays,) [1]. This data article describes (1) the synthesis and spectral ch...

Glucosinolates are a class of secondary metabolites with important roles in plant defense and human nutrition. To uncover regulatory mechanisms of glucosinolate production, we screened Arabidopsis thaliana T-DNA activation-tagged lines and identified a high-glucosinolate mutant caused by overexpression of IQD1 (At3g09710). A series of gain- and loss-of-function IQD1 alleles in different accessi...

The first total synthesis of 4-methylthio-3-butenyl glucosinolate (MTBG), a natural bioactive compound and a precursor of radish phototropism-regulating substances, was achieved from commercially available 1,4-butanediol. The glucosinolate framework was prepared by coupling of an oximyl chloride derivative and tetraacetyl thioglucose. A methylthio group was introduced to the framework by a Witt...

The defense-related plant metabolites known as glucosinolates play important roles in agriculture, ecology, and human health. Despite an advanced biochemical understanding of the glucosinolate pathway, the source of the reduced sulfur atom in the core glucosinolate structure remains unknown. Recent evidence has pointed toward GSH, which would require further involvement of a GSH conjugate proce...

In order to investigate the changes in fatty acids composition of rapeseed lines under Chitosan application, a factorial split-plot experiment was conducted based on RCBD in 3 replications during 2 years (2014-2016) in Karaj, Iran. Irrigation and Chitosan as main plots and cultivar in sub-plots were considered. In this study, treatmen...

Germ-free growing rats were fed on a glucosinolate-rich diet (rapeseed-meal-based) and compared with counterparts fed on a glucosinolate-free diet (soya-bean-meal-based), both diets being isonitrogenous and isoenergetic. For each diet half the animals received phenobarbital in drinking water as an inducer of xenobiotic-metabolizing enzymes. Some of the usual deleterious glucosinolate-linked eff...

Glucosinolates and their breakdown products have been recognized for their effects on plant defense, human health, flavor and taste of cruciferous vegetables. Despite this importance, little is known about the regulation of the biosynthesis and degradation in Brassica rapa. Here, the identification of quantitative trait loci (QTL) for glucosinolate accumulation in B. rapa leaves in two novel se...

Most phenotypic variation present in natural populations is under polygenic control, largely determined by genetic variation at quantitative trait loci (QTLs). These genetic loci frequently interact with the environment, development, and each other, yet the importance of these interactions on the underlying genetic architecture of quantitative traits is not well characterized. To better study h...

The development of low erucic acid, low glucosinolate cultivars of canola seed has led to the availability of a feed ingredient with considerable potential to replace soyabean meal in diets for all classes of farm animals. The sinapine and glucosinolate content of various canola cultivars cultivated in two areas of the Western Cape, South Africa were compared. There were no significant differen...