OBJECTIVES The primary role of γ-glutamyltransferase is to metabolize reduced glutathione by assimilating and utilizing precursor amino acids for intracellular glutathione synthesis. The aim of this study is to investigate the exact role of γ- glutamyltransferase enzyme in the oxidative stress associated with menopause. METHODS This study was carried out at the Chemistry and Biochemistry depa...

The activity of y-glutamyltransferase (EC 2.3.2.2) is measured on the DuPont aca by an adaptation of the Szasz procedure ;-y-glutamyl-p-nitroanilide is transferred to glycylglycine, thereby releasing p-nitroaniline, which is measured at 405 nm. I have noticed low glutamyltransfer-ase activity in patients with cholestasis wherealkaline phosphate (EC 3.1.3.1) is increased, total bilirubin> 100 mg...

The three brush-border enzymes--alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), and gamma-glutamyltransferase (EC 2.3.2.2)--are present in the urine of healthy persons in two variants, a particulate form and a soluble one. They can be separated by electrophoresis in agarose gel and by ultracentrifugation. The particulate forms exhibit similar electrophoretic mobility, b...

The formation of y-glutamylhydroxamate by homogenates under optimum assay condition showed an inconstancy in the ratios of the enzyme activities utilizing L-glutamate and ATP (y-glutamylhydroxamate synthetase) and L-glutamine and ADP (L-glutaminehydroxylamine glutamyltransferase) in a number of normal and neoplastic rat tissues. Although y-glutamylhydroxamate synthetase activities in adult live...

Passive immunity in lambs: Colostral and serum γ-glutamyltransferase as a predictor of IgG concentration related to the diseases from birth 12 weeks life | E Gokce, AH Kirmizigul, O Atakisi, M Kuru, HM Erdogan Agricultural Journals

Gamma-glutamyltransferase activity in serum is shown to be competitively inhibited by the two substrates gamma-glutamyl-4-nitroanilide and glycylglycine. Awareness of this is of importance when one is choosing final reaction conditions for the assay of the enzyme. Gamma-glutamyltransferase probably acts by a "ping-pong bi-bi" kinetic mechanism, which fits with the double competitive substrate i...

Serum y-glutamyltransferase (EC 2.3.2.2) showed microheterogeneity on electrofocusing, owing to variations in sialic acid content. We investigated the isoform patterns of papaintreated serum samples on agarose gels containing nonionic detergent and ampholytes in the low pH range. Serum from cases of cholestasis show seven bands with y-glutamyltransferase activity. These same bands were also fou...

BACKGROUND Failure of passive transfer of maternal immunity via colostrum can occur in the bovine, and a number of blood tests have been developed to test calves for this failure. It is not clear which test is most suitable for this purpose. The objective was to examine the most commonly used tests for failure of passive transfer and to decide which is most suitable for routine laboratory use. ...

Carcinoembryonic antigen and activities of glucosephosphate isomerase (EC 5.3.1.9), y-glutamyltransferase (EC 2.3.2.2), and lactate dehydrogenase (EC 1.1.1.27) were measured in aqueous extracts of fetal, normal adult, and malignant human colon tissues. Fetal colon, as well as primary and metastatic colon tumor tissue, showed higher activities of these analytes than did normal adult human colon....