Glutathione (GSH), a tripeptide (L γ-glutamyl-cysteinyl-glycine) is the major nonprotein thiol found abundantly in eukaryotic cells (1–3). GSH plays a critical role in many metabolic pathways. It acts as a cofactor for intracellular enzymes like the glutathione S-transferases (GST) and glutathione peroxidase; it aids in amino acid transport and is essential for detoxification of xenobiotics and...

Bangia fuscopurpurea is a widespread intertidal seaweed that commercially cultured in China. This frequently exposed to hyposalinity stress, but little known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles many organisms under variety of abiotic including hyposaline stress. In this study, we investigated response key metabolites and enzymes involved ...

PURPOSE To characterize synthesis and transport of glutathione (GSH) by cultured human retinal pigment epithelial (RPE) cells. METHODS Cultured human RPE cells were depleted of glutathione, then incubated with various concentrations of cysteine, glutamate, and glycine or with glutathione. High-performance liquid chromatography was used to measure intracellular glutathione with time. RESULTS...

The glutathione content and the activities of several enzymes in its metabolism, glutathione reductase, glutathione peroxidase and γ-glutamyl transpeptidase, were assayed in uteri obtained from estrogen-treated rats and in R3230AC mammary adenocarcinomas obtained from ovariectomized, intact and estrogen-treated hosts. Normal mammary glands, obtained 10–12 days post-partum, were also examined fo...

Kombu RS, Zhang GF, Abbas R, Mieyal JJ, Anderson VE, Kelleher JK, Sanabria JR, Brunengraber H. Dynamics of glutathione and ophthalmate traced with H-enriched body water in rats and humans. Am J Physiol Endocrinol Metab 297: E260–E269, 2009. First published April 28, 2009; doi:10.1152/ajpendo.00080.2009.—We developed a LC-MS-MS assay of the H labeling of free glutathione (GSH) and bound glutathi...

This work is the next step in studying interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed our group) and glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed concentration- pH-dependent GSH conjugation of rat liver microsomes cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found various tumors. The f...

Kombu RS, Zhang GF, Abbas R, Mieyal JJ, Anderson VE, Kelleher JK, Sanabria JR, Brunengraber H. Dynamics of glutathione and ophthalmate traced with H-enriched body water in rats and humans. Am J Physiol Endocrinol Metab 297: E260–E269, 2009. First published April 28, 2009; doi:10.1152/ajpendo.00080.2009.—We developed a LC-MS-MS assay of the H labeling of free glutathione (GSH) and bound glutathi...

When drought occurs during the maize-filling period, probability of yield decline increases. Abscisic acid (ABA) plays a regulatory role in physiological and metabolic activities plant development. However, its effect on antioxidant system maize leaves grain-filling stage is unclear. Maize plants (Zhengdan958) were used as an experimental material, ABA was sprayed stage. The placed under condit...