1. Studies were performed to investigate the metabolic fate of dipeptides when administered intravenously in rats. Glycyl-leucine, glycylglycine or glycylsarcosine was injected into the jugular vein. The plasma disappearance rate after the peak plasma concentrations was most rapid for glycyl-leucine and least rapid for glycylsarcosine. 2. During urine collection for 40 min, trace amounts of gly...

y-Glutamyltransferase (EC 2.3.2.2) from human liver, kidney, pancreas, and duodenum migrated in acrylamide gels (65 g/L) as a single band with the following decreasing order of electrophoretic mobility: liver > pancreas > kidney > duodenum. The initial velocity kinetic constants of pancreatic and duodenal y-glutamyftransferase and of the enzyme in human serum were determined and compared with t...

* For DPN synthesis, 1.0 ml. of reaction mixture contained 50 y of the enzyme preparation, 0.3 micromole of MgCl 2, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to ATP and NMN; for ATP synthesis 1.0 ml. contained 50 y of the enzyme preparation, 0.75 micromole,of MgC12, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to DPN and P-P. Constant values were reached a...

Kinetic Controlled Chirality Transfer and Induction Two-dimensional hydrogen-bonding assemblies with different chirality, e.g. achiral, homochiral racemic, are synthesized on Au(111) surface by using achiral glycylglycine, one of the simplest polypeptides, as building blocks. induction related symmetry broken in introduced handedness assembled motifs then amplified to entire via interaction bet...

We propose that a chemically agnostic approach to explore the origin of life, using an automated recursive platform based on droplet microfluidics, could be used to induce artificial chemical evolution by iterations of growth, speciation, selection, and propagation. To explore this, we set about designing an open source prototype of a fully automated evolution machine, comprising seven modules....