نتایج جستجو برای: gp60
تعداد نتایج: 146 فیلتر نتایج به سال:
Caveolae-mediated endocytosis in endothelial cells is stimulated by the binding of albumin to gp60, a specific albumin-binding protein localized in caveolae. The activation of gp60 induces its cell surface clustering and association with caveolin-1, the caveolar-scaffolding protein. This interaction leads to Gi-induced Src kinase activation, which in turn signals dynamin2-mediated fission and d...
We determined the concentration dependence of albumin binding, uptake, and transport in confluent monolayers of cultured rat lung microvascular endothelial cells (RLMVEC). Transport of (125)I-albumin in RLMVEC monolayers occurred at a rate of 7.2 fmol. min(-1). 10(6) cells(-1). Albumin transport was inhibited by cell surface depletion of the 60-kDa albumin-binding glycoprotein gp60 and by disru...
سابقه و هدف: کریپتوسپوریدیوم تک یاختهای کوچک از کوکسیدیاها است که دامنه گستردهای شامل انسان، حیوانات اهلی و وحشی را آلوده میکند. از آنجاییکه هیچ اطلاعاتی از ساب تایپهای ایزولههای جداشده از ایران وجود ندارد، هدف این مطالعه انگشتنگاری DNA ایزولههای جدا شده از گاوهای آلوده در استان قزوین بر اساس بررسی توالی به دست آمده از ژن GP60 بود. مواد و روش ها: در این مطالعه مجموعاً 25 ایزوله کر...
The purpose of this study was to investigate the occurrence Cryptosporidium infection and potential for transmission spp. between animals humans in northern Vietnam. A total 2715 samples (2120 human diarrheal samples, 471 non-diarrheal 124 animal stool samples) were collected through our community survey an agricultural area. All tested by direct immunofluorescence assay (DFA) using a fluoresce...
Cryptosporidium ubiquitum is an emerging zoonotic pathogen. In the past, it was not possible to identify an association between cases of human and animal infection. We conducted a genomic survey of the species, developed a subtyping tool targeting the 60-kDa glycoprotein (gp60) gene, and identified 6 subtype families (XIIa-XIIf) of C. ubiquitum. Host adaptation was apparent at the gp60 locus; s...
Few studies have examined the molecular epidemiology of cryptosporidiosis in developing countries. In this study, DNA of 69 microscopy-positive human fecal samples collected from Malawi were examined by multilocus genetic analyses. From 43, 27 and 28 of the samples, the SSU rRNA, 70 kDa heat shock protein (HSP70) and 60 kDa glycoprotein (GP60) genes, respectively, were successfully PCR-amplifie...
introduction: cryptosporidium is an apicomplexa parasite that infects humans and a wide range of domestic and wild animals. however, the sub-genotypes of the species infecting animals in iran are unclear. the aim of the present study isto identify dna fingerprinting of bovine cryptosporidium in qazvin province using sequences of gp60 gene. materials and methods : in this study we investigated...
The gene product 60 (gp60) of bacteriophage T4 is synthesized as a single polypeptide chain from a discontinuous reading frame as a result of bypassing of a non-coding mRNA region of 50 nucleotides by the ribosome. To identify the minimum set of signals required for bypassing, we recapitulated efficient translational bypassing in an in vitro reconstituted translation system from Escherichia col...
background: cryptosporidium is a worldwide protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. the aim of the present study was determination of subtypes of cryptosporidium among children with diarrhea in tehran by sequence analysis of the highly polymorphic 60-kda glycoprotein ( gp60 ) gene. methods: fecal samples were collected from 794 diarr...
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