نتایج جستجو برای: hansenula polymorpha

تعداد نتایج: 1687  

2016
Yeganeh Talebkhan Tannaz Samadi Armin Samie Farzaneh Barkhordari Mohammad Azizi Vahid Khalaj Esmat Mirabzadeh

BACKGROUND AND OBJECTIVES During past decades Hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. MATERIALS AND METHODS The corresponding nucleotide sequences for the expression of heterologous genes in Hansenula poylmorpha were extracted and as...

Journal: :FEMS yeast research 2007
Olena G Stasyk Tim van Zutphen Huyn Ah Kang Oleh V Stasyk Marten Veenhuis Andriy A Sibirny

In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficiency in the putative H. polymorpha homologues of transcriptional repressors Mig1 (HpMig1 and HpMig...

Journal: :FEMS yeast research 2007
Loknath Gidijala Ida J van der Klei Marten Veenhuis Jan A K W Kiel

We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required. To this end, we constructed a novel host-vector system based on methionine auxotrophy and the H. polymorpha MET6 gene, which encodes a putative cystathionine be...

Journal: :Yeast 1999
J A Kiel K B Rechinger I J van der Klei F A Salomons V I Titorenko M Veenhuis

Via functional complementation we have isolated the Hansenula polymorpha PDD1 gene essential for selective, macroautophagic peroxisome degradation. HpPDD1 encodes a 116 kDa protein with high similarity (42% identity) to Saccharomyces cerevisiae Vps34p, which has been implicated in vacuolar protein sorting and endocytosis. Western blotting experiments revealed that HpPDD1 is expressed constituti...

Journal: :FEBS letters 1993
G J Sulter H R Waterham E G Vrieling J M Goodman W Harder M Veenhuis

A 47 kDa integral peroxisomal membrane protein (PMP47) of Candida boidinii was expressed in wild type (WT) and a temperature-sensitive (Ts6) peroxisome-deficient (per) mutant of Hansenula polymorpha. The subcellular location of PMP47 appeared to be dependent on the level of expression. At low expression levels PMP47 was sorted to the peroxisomal membrane; however, in Ts6 cells grown at restrict...

Journal: :Applied and environmental microbiology 2012
Christian Dusny Frederik Sven Ole Fritzsch Oliver Frick Andreas Schmid

Singularized cells of Pichia pastoris, Hansenula polymorpha, and Corynebacterium glutamicum displayed specific growth rates under chemically and physically constant conditions that were consistently higher than those obtained in populations. This highlights the importance of single-cell analyses by uncoupling physiology and the extracellular environment, which is now possible using the Envirost...

Journal: :Biochimica et biophysica acta 2010
Ruchi Saraya Małgorzata N Cepińska Jan A K W Kiel Marten Veenhuis Ida J van der Klei

In budding yeast Saccharomyces cerevisiae, the peroxisomal protein Inp2 is required for inheritance of peroxisomes to the bud, by connecting the organelles to the motor protein Myo2 and the actin cytoskeleton. Recent data suggested that the function of Inp2 may not be conserved in other yeast species. Using in silico analyses we have identified a weakly conserved Inp2-related protein in 18 spec...

Journal: :FEBS letters 2009
Elena Kurbatova Marleen Otzen Ida J van der Klei

Emp24 is a member of the p24 protein family, which was initially localized to the endoplasmic reticulum, Golgi and COP vesicles, but has recently shown to be associated with Saccharomyces cerevisiae peroxisomes as well. Using cell fractionation and electron- and fluorescence microscopy, we show that in the yeast Hansenula polymorpha, Emp24 also associates with peroxisomes. In addition, we show ...

Journal: :Applied and environmental microbiology 2012
Juan Borrero Gotthard Kunze Juan J Jiménez Erik Böer Loreto Gútiez Carmen Herranz Luis M Cintas Pablo E Hernández

The bacteriocin enterocin A (EntA) produced by Enterococcus faecium T136 has been successfully cloned and produced by the yeasts Pichia pastoris X-33EA, Kluyveromyces lactis GG799EA, Hansenula polymorpha KL8-1EA, and Arxula adeninivorans G1212EA. Moreover, P. pastoris X-33EA and K. lactis GG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities...

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