high levels of regulated oncogen-alpha (gro-a) expression have been observed in the liver. gro-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. in this study, hepatocytes (2×106 cell/ml) were...

it has been hypothesized that the survival, development, fecundity and population of insects are affected significantly by high temperatures. the diamondback moth, plutella xylostell (l.) (lepidoptera: plutellidae), is a serious and economically important pest of cruciferous crops throughout the word. in this research, the adult longevity and fecundity of p. xylostella were studied in laborator...

As all biologists appreciate, there is constant interaction between life and the environment, and temperature plays a critical role. It establishes distribution limits, it affects rates of function as well as the survival of organisms. Notoriously sensitive to temperature is plant growth. Critical steps in plant life such as germination, flowering and breaking of dormancy can be manipulated by ...

recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...

Triploidy in grass carp, Ctenopharyngodon idella Valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. Two simplified methods explained for verification of triploidy in grass carp. The cold shock (7 ?C) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. In cold shock, the start point (2.0 min after fertilization) showed th...

background permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. ion channel m2 protein is conserved among all influenza a viruses, allowing the virus to enter host cells. objectives to prepare an e...

background: wet cupping therapy is a complementary therapy in pain management. the mechanism of this therapy, however, needs further elucidation. cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (hsp70). its benefit in pain reduction could be mediated by the expression of ß-endorphin. this study aimed at determining the correlation between hsp70 and...

luteolin, as a natural polyphenolic compound, has neuroprotective effect and exerts its function by attenuation of apoptosis and oxidative stress factors. emerging evidences indicate that oxidative stress leads to neurodegeneration but is not the initial event and endoplasmic reticulum stress (er) is often considered to be the stimulus event which is caused by accumulating of misfolded proteins...

background and objectives: the purpose of the present study was to investigate effects of various heat shock conditions and fast freezing and subsequent thawing on the viability and recovery of bacillus coagulans and bacillus subtilis as probiotic sporeformers, and also to compare spore plate and microscopic counts. materials and methods: after preparing the final suspensions of b. coagulans an...