Abelmoschus esculentus (Okra) is used in the traditional treatment of cancer, hyperlipidaemia and hyperglycaemia. We, therefore, investigated its composition potential cytotoxic or antioxidant properties that might underlie phytotherapeutic applications. Its methanolic fruit extract yielded compounds 1, 2 3, identified through NMR, UV MS analyses as olean-12-en-3-O-β-d-glucopyranoside, isoquerc...

background: the exposure of cell lines to low-dose irradiation leads to changes at molecular level, which may induce adaptive response. we examined radio-adaptive doses effect on human colorectal adenocarcinoma cell line (ht29) and human fetal fibroblasts (mrc5) cell line followed by hyper and hypo fractionation regimes, with main purpose to decrease cell viability in ht29, and at the same time...

Background: Cervical cancer is the second most common female cancer worldwide. Inhibitors of apoptosis proteins (IAPs) block apoptosis; therefore, therapeutic strategies targeting IAPs have attracted the interest of researchers in recent years. Apollon, a member of IAPs, inhibits apoptosis and cell death. RNA interference is a pathway in which small interfering RNA (siRNA) or shRNA (short hairp...

introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...

background: our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on mda-mb231 and hl-60 cell lines, through induction of apoptosis. the purpose of this study was to investigate apoptosis-induced mechanism on hela and mrc-5 cells by icd-85 through activation of caspase-8. methods: cell viability, cytosolic enzyme lactate dehydrogenase (ldh) and cell morphology we...

based on the evaluation of two human cell lines, hela and chang, abeuploidy and several marker chromosomes were found in both cells. the morphological characteristic of marker chromosomes of chang cells was distinctly different from hela. certain submetacentric marker chromosome was frequently present among 80% of marker chromosomes of chang cells which distinguished this line from hela, which ...

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

Estradiol valerate (Exogenous estrogen) is a synthetic ester and is also a female estrogen hormone. There arestudies showing that estradiol may have anticancer effects. This study was performed to reveal the effects ofexogenous estrogens on the viability of cervical cancer cells in cell culture. We used Hela cells as our cell linein this study. Hela cells were exposed to 0.0001, 0.001, 0.01, 0....

background: essential oils are found to have multiple active components which can show in vitro cytotoxic action against various cancerous cell lines. this study reports the in vitro cytotoxic effects of the essential oil from artemisia absinthium l. (asteraceae) growing wild in iran. methods: water-distilled essential oil of a. absinthium collected from ardabil, north-western iran, was examine...

There are a sub-population of cells in tumor tissues known as cancer stem cells (CSCs) which have similar features with stem cells, including self-renewal and differentiation capacity. Recently, it was established that not only stem cells factors such as Oct4, but also ES-associated lncRNAs are contributing to various regulatory aspects of CSCs. Myocardial infarction associated transcript (MIAT...