A single radial hemolysis assay for parainfluenza-3 virus antibody is described as a simple, sensitive, accurate, and precise alternative to hemagglutination inhibition and serum neutralization tests. A highly significant correlation exists between single radial hemolysis zone areas and hemagglutination inhibition titers of both serum and nasal secretion samples. Antibody conversions equivalent...

background and aims: influenza a virus infects birds and some mammalian including human. h1 and h3 subtypes are circulated in both human and birds’ population. to determine the prevalence of the mentioned subtypes in birds, different captive bird species in tehran zoo, and affiliated centers in tehran were investigated for virus infection. methods: in this study, between november 2008 and febru...

A sensitive immunoassay procedure for the detection of the thrombopoietic-stimulating factor (TSF) has been developed. This procedure Is similar to the hemagglutination-inhibition (HAl) technique that is available for erythropoietin. For the production of TSF, sheep were made thrombocytopenic by the Injection of antiplatelet serum. TSF from sheep sera was partially purified by column chromatogr...

Three assays for detection of rubella antibodies, Rubella G (fluorescence immunoassay [FIA]), Rubacell (passive hemagglutination), and Rubaquick (passive hemagglutination with rotation), were compared with hemagglutination inhibition. A total of 100 serum specimens were selected, 68 of which had an FIA value of less than or equal to 25. On initial testing, among the four tests, there was agreem...

Various concentrations of pig, guinea pig, chicken, and turkey erythrocytes (RBCs) were cryopreserved and compared with fresh RBCs in hemagglutination and hemagglutination inhibition tests. Hemagglutination and hemagglutination inhibition titers obtained with cryopreserved or fresh RBCs from the same donor differed by not more than one twofold dilution. RBCs cryopreserved at a concentration of ...

conclusions the aiv loaded into cnp vaccines induce appropriate antibody titers, after a single immunization, while requiring a low dose of antigen. the cnp also represent an interesting new platform for antigen delivery and a promising adjuvant candidate for h9n2 inactivated influenza vaccine. results the cnp did not cause any mortality or side effects, when chickens were administered the prep...

A passive hemagglutination inhibition assay was studied by using a hyperimmune serum from rabbits immunized with whole yeast cells (Candida albicans group A). This technique was effective at detecting small amounts of laboratory-prepared mannan or a whole-cell extract of C. albicans. Of 32 patients with documented disseminated candidiasis that were tested, 19 showed evidence of circulating anti...