Mutualistic association between entomopathogenic Photorhabdus bacteria and Heterorhabditis nematodes represents one of the emerging model systems in symbiosis studies, yet little is known about this partnership from a coevolutionary perspective. Herein, we investigated phylogenetic and cophylogenetic relationships of Heterorhabditis and Photorhabdus strains using molecular markers Internal Tran...

Here, we report the draft genome sequence of Photorhabdus heterorhabditis strain VMG, a symbiont of the entomopathogenic nematode Heterorhabditis zealandica in South Africa. The draft genome sequence is 4,878,919 bp long and contains 4,023 protein-coding genes. The genome assembly contains 262 contigs with a G+C content of 42.22%.

A survey of entomopathogenic nematodes was conducted in the north Pacific (Guanacaste Conservation Area) and southeast Caribbean (Gandoca-Manzanillo Natural Refuge) regions of Costa Rica. Out of a total of 41 soil samples, 5 were positive for entomopathogenic nematodes (20.5%), with 3 (12.3%) containing Steinernema and 2 (8.2%) Heterorhabditis isolates. Morphological and molecular studies were ...

In the present study, the entomopathogenic nematodes were recorded from 89 samples (5.37%) out of 1656 samples collected from 20 sites representing ecologically diverse types of habi ta ts in Meghalaya, India . Out of 89 positive samples, the frequency of occurrence of Steinernema spp. was found to be more (73.03%) than Heterorhabditis sp. (26.97%). All the EPN positive samples were from the fo...

The potential for entomopathogenic nematodes to control ßies in cattle feedlots was determined by screening 40 strains representing 8 species of Heterorhabditis Poinar and 5 species of Steinernema Travassos for virulence toward 3rd-instar house ßies (maggots), Musca domestica L. None of the 22 strains of Heterorhabditis infecting maggots caused signiÞcant levels of mortality in a Þlter paper as...

The potential for entomopathogenic nematodes to control ßies in cattle feedlots was determined by screening 40 strains representing 8 species of Heterorhabditis Poinar and 5 species of Steinernema Travassos for virulence toward 3rd-instar house ßies (maggots), Musca domestica L. None of the 22 strains of Heterorhabditis infecting maggots caused signiÞcant levels of mortality in a Þlter paper as...

The ability of Steinernema feltiae or Heterorhabditis bacteriophora infective juveniles (IJ), when applied to the soil surface, to infect a Galleria mellonella larva at the base of a soil-filled cup (276 cm(3)) was evaluated in the presence and absence of 100 larvae of a non-target insect, the aphid midge Aphidoletes aphidimyza, near the soil surface. In all four trials with either S. feltiae o...

The susceptibility of all nymphal and adult stages of the Acridid grasshopper H. littoralis to different inoculum levels of the insect pathogenic nematodes of the family Heterorahabitidae was recorded. The two tested nematode species were local isolates belong to Heterorhabditis indica (Poinar, Karunakar & David, 1992) (isolate EGB4) and Heterorhabditis bacteriophora (Poinar, 1979) (isolate EKB...

xenorhabdus and photorhabdus are members of the family enterobacteriaceae, and are engaged in a mutualistic association with the entomopathogenic nematodes, steinernema and heterorhabditis, respectively. in the present study, the organic fraction of ethyl acetate bacterial crude extracts of the symbiotic bacteria, xenorhabdus stockiae and photorhabdus luminescens isolated from entomopathogenic ...

The persistence of Heterorhabditis megidis in soil was studied over a 4-week period. On days 0, 2, 14, and 28, infective juveniles (IJ) were extracted by centrifugal flotation, Baermann funnel, and baiting of soil with Tenebrio molitor larvae, which were then dissected. Extraction efficiencies on day 0 were 82% by centrifugal flotation, 56% by Baermann funnel, and 19.8% by bait insect. The rela...